VEGF和NOS在涎腺腺样囊性癌中的表达及与细胞增殖的关系

目的研究涎腺腺样囊性癌(salivary adenoid eystic carcinoma,SACC)组织血管内皮生长因子(vascular endothelial growth factor,VEGF)、诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)的表达与癌细胞增殖的相关性。方法应用免疫组化方法检测32例SACC手术切除标本VEGF、iNOS、eNOS和增殖细胞核抗原(proliferating cell unclear angigen,PCNA)的分布及表达。结果①20例(62.5%)SACC组织表达VEGF,22例(68.75%)表达iNOS;25例(78.13%)表达eNOS;②VEGF与iNOS的表达具有明显相关性,VEGF与eNOS的表达无明显相关性;③表达VEGF的涎腺腺样囊性癌PCNA标记指数(PLI)明显高于不表达VEGF的SACC;表达iNOS的涎腺腺样囊性癌PLI明显高于不表达iNOS的SACC。表达eNOS的涎腺腺样囊性癌PLI与不表达eNOS的SACC无显著性差异(P>O.05)。结论①VEGF与iNOS的表达具有明显相关性,说明iNOS在VEGF的生成和发挥作用过程中起重要作用;②PLI随着VEGF和iNOS表达的增加而增加;说明二者对SACC细胞增殖具有促进作用。

Correlation of carcinoma cell proliferation and expression of VEGF and NOS in salivary adenoid cystic carcinoma

Objective To study the relation of protein expression of inducible nitric oxide synthase(iNOS),endothelial nitric oxide synthase(eNOS) and Vascular endothelial growth factor(VECF) as related to salivary adenoid cystic carcinoma(SACC) proliferation.Methods SACC tissue specimens were obtained from 32 patients undergoing operation,by using the immunohistochemisry technique,the expression of iNOS,eNOS and VEGF and the expression and distribution of PCNA(proliferating ce11 unclear angigen,PCNA) were studied.Results ①Positive VEGF and iNOS and eNOS immunostaining were detected in 20(62.5%),22(68.75%) and 25(78.13%) patients with SACC respectively.②Expression of VEGF bore a significant positive relation to iNOS,but not eNOS.③PLI(PCNA labelling index) in VEGF and iNOS positive case was significantly higher than that in VEGF and iNOS negative cases.No difference PLI level was noted in both eNOS positive and negative cases.Conclusion ①VEGF and iNOS bear a significant positive correlationship in SACC,showing that iNOS,rather than eNOS,plays an important role in VEGF production.②PLI increased with the increment of VEGF and iNOS expression.VEGF and iNOS are known to enhance SACC cell proliferation.