| The expression of CD40 and CD40L on the surface of peripheral blood mononuclear cells in asthmatic rats and the effect of anti-CD40L McAb on Th1 and Th2 cytokines |
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| 作者姓名： | Keying Xue Shengdao Xiong Weining Xiong Yongming Zhou Xiang Long Yan Li |
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| 作者单位： | [1]Department of Respiratology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China [2]Department of Research and Applying Stem Cell Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 ,China |
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| 摘 要： | Objective： To investigate the expression of CD40 and CD40 ligand （CD4OL） on the surface of peripheral blood mononuclear cells（PBMCs） in asthmatic rats and the effect of anti-CD40L McAb on cytokines of PBMCs. Methods： Flow cytometry and RT-PCR were used to detect the expression of CD40 and CD40L of PBMCs in asthmatic rats. After the PBMCs was treated with anti-CD40L McAb, ELISA was used to detect the IL-4 and IFN-γ levels of culture supernatants. Results： Compared with the normal control group, the expression of CD40 and CD40L of PBMCs in asthmatic rats increased （P 〈 0.05）. Compared with the untreated group, the level of IL-γ and the ratio of IL-4/IFN-γ decreased after the PBMCs was treated with anti-CD40L McAb（P 〈 0.05）. Conclusion： The expression of CD40 and CD40L on the surface of PBMCs in asthmatic rats was up-regulated. Anti-CD40L McAb can rectify the imbalance of Th1 and Th2 cytokines.
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| 关 键 词： | 基因表达 外周血 单核细胞 哮喘 细胞活素 |
| 收稿时间： | 2006-10-23 |
The expression of CD40 and CD40L on the surface of peripheral blood mononuclear cells in asthmatic rats and the effect of anti-CD40L McAb on Th1 and Th2 cytokines |
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| Keying Xue,Shengdao Xiong,Weining Xiong,Yongming Zhou,Xiang Long,Yan Li.The expression of CD40 and CD40L on the surface of peripheral blood mononuclear cells in asthmatic rats and the effect of anti-CD40L McAb on Th1 and Th2 cytokines[J].Acta Universitatis Medicinalis Nanjing,2006,20(3):162-165. |
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| Authors: | Keying Xue Shengdao Xiong Weining Xiong Yongming Zhou Xiang Long Yan Li |
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| Abstract: | Objective: To investigate the expression of CD40 and CD40 ligand (CD40L) on the surface of peripheral blood mononuclear cells(PBMCs) in asthmatic rats and the effect of anti-CD40L McAb on cytokines of PBMCs. Methods: Flow cytometry and RT-PCR were used to detect the expression of CD40 and CD40L of PBMCs in asthmatic rats. After the PBMCs was treated with anti-CD40L McAb, ELISA was used to detect the IL-4 and IFN-γ levels of culture supernatants. Results: Compared with the normal control group, the expression of CD40 and CD40L of PBMCs in asthmatic rats increased (P ＜ 0.05). Compared with the untreated group, the level of IL-4 and the ratio of IL4/IFN-γ decreased after the PBMCs was treated with anti-CD40L McAb(P ＜ 0.05). Conclusion: The expression of CD40 and CD40L on the surface of PBMCs in asthmatic rats was up-regulated.Anti-CD40L McAb can rectify the imbalance of Th1 and Th2 cytokines. |
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| Keywords: | asthmatic rats peripheral blood mononuclear cells CD40 CD40L anti-CD40L McAb cytokine |
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