| 全反式维甲酸对肺癌胸腔种植转移动物模型的干预作用 |
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| 引用本文： | 赵斌斌,朱运奎,肖永久,李继东,刘卫,王晓芹.全反式维甲酸对肺癌胸腔种植转移动物模型的干预作用[J].现代肿瘤医学,2010,18(1). |
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| 作者姓名： | 赵斌斌 朱运奎 肖永久 李继东 刘卫 王晓芹 |
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| 作者单位： | 1. 兰州军区兰州总医院呼吸内科,甘肃,兰州,730050;兰州大学临床医学院,甘肃,兰州,730050
2. 兰州军区兰州总医院呼吸内科,甘肃,兰州,730050 |
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| 摘 要： | 目的:建立裸鼠非小细胞肺癌转移模型,研究全反式维甲酸(all-trans retinoic acid ,ATRA)对肺癌转移模型生长和转移情况的影响.探讨裸鼠血清中MMP-2,9活性水平和ATRA干预对MMP-2,9活性的影响.方法:通过右侧胸腔注射接种非小细胞肺癌H460细胞制作肺癌裸鼠胸腔种植转移模型,建模后给予ATRA干预.结果:接种H460细胞后所有动物均出现肺部肿瘤,肿瘤扩散到临近胸腔和远处腹腔器官,与人体病理情况一致.ATRA干预后,胸腔内的肿瘤质量(0.43±0.12)低于对照组(0.96±0.18),(P＜0.05),肝平均转移灶数目和转移发生率分别为(0.08±0.28)个, 8%低于对照组(4.67±4.47)个,58%,(P＜0.05). 胰腺平均转移灶数目和转移发生率分别为(0.67±1.50)个,25%低于对照组 (9.00±7.68)个,75%,(P＜0.05).后腹膜转移灶数目(6.42±4.33)个低于对照组(23.00±8.75)个,(P＜0.05),转移发生率(83%)低于对照组(100%),但无统计学意义(P＞0.05).造模后血清中MMP-2,9活性增高,ATRA干预后MMP-2活性明显下降.结论:ATRA干预能抑制肺癌H460转移模型原位肿瘤生长和远隔器官的转移.血清中MMP-2,9可作为肺癌诊断和做为判断治疗效果的有用分子标记物.
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| 关 键 词： | 全反式维甲酸 肺癌 转移 基质金属蛋白酶 动物模型 |
Inhibiting effects of all-trans retinoic acid on a model implanted in the pleural cavity of nude mice |
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| ZHAO Bin-bin,ZHU Yun-kui,XIAO Yong-jiu,LI Ji-dong,LIU Wei,WANG Xiao-qin.Inhibiting effects of all-trans retinoic acid on a model implanted in the pleural cavity of nude mice[J].Journal of Modern Oncology,2010,18(1). |
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| Authors: | ZHAO Bin-bin ZHU Yun-kui XIAO Yong-jiu LI Ji-dong LIU Wei WANG Xiao-qin |
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| Institution: | ZHAO Bin-bin1,2,ZHU Yun-kui1,XIAO Yong-jiu1,LI Ji-dong1,LIU Wei1,WANG Xiao-qin1 1Department of Respiratory Medicine,Lanzhou General Hospital of Lanzhou Military Area Comm, of Chinese PLA,Lanzhou 730050,2 Clinical Medical College,Lanzhou University,China. |
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| Abstract: | Objective:To establish a metastatic model of human non-small cell lung carcinoma(NSCLC)and investigate the influence of treated all-trans retinoic acid(ATRA)about growth and metastasis on the metastasis model of lung caner;to explore activity of MMP-2,9 in the serum of nude mice and the possible effect on the activity of MMP-2,9 after treatment of ATRA.Methods:The metastasis model of lung cancer was established by injection of non-small cell lung carcinoma line H460 cells into the right pleural space of nud... |
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| Keywords: | all-trans retinoic acid lung caner metastasis matrix metalloproteinase animal model |
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