| 可磁化固相二抗分离技术在放射免疫分析中的通用性探讨 |
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| 引用本文： | 刘慎沛,崔武任,李建华,叶维新,方唯一,魏文华.可磁化固相二抗分离技术在放射免疫分析中的通用性探讨[J].华中科技大学学报(医学版),1989(1). |
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| 作者姓名： | 刘慎沛 崔武任 李建华 叶维新 方唯一 魏文华 |
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| 作者单位： | 同济医科大学实验医学研究中心核医学研究室
(刘慎沛，崔武任，李建华，叶维新)，同济医科大学附属同济医院放射免疫室
(方唯一)，同济医科大学附属同济医院放射免疫室(魏文华) |
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| 摘 要： | 采用本室建立的可磁化固相二抗 (DAMP) 分离技术与常规应用的双抗体法,聚乙二醇(PEG)法、活性炭法对10种RIA药盒进行实验比较。结果显示DAMP法与双抗体法的五项质控指标均在RIA质控标准的最佳范围内,明显优于PEG法与活性炭法,表明DAMP法具有双抗体法的分离效果,且较双抗体法更为简便、快速,适合用作RIA的通用分离方法。
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| 关 键 词： | 放射免疫分析(RIA) 分离技术 质量控制 |
Determination of Universality of Separation Technique Based on Solid Phase Double Antibody Magnitizable Particle in Radioimmunoassay |
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| Liu Shenpei,Cui Wuren,Li Jianhua,Ye Weixin.Determination of Universality of Separation Technique Based on Solid Phase Double Antibody Magnitizable Particle in Radioimmunoassay[J].Journal of Huazhong University of Science and Technology(Health Sciences),1989(1). |
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| Authors: | Liu Shenpei Cui Wuren Li Jianhua Ye Weixin |
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| Institution: | Liu Shenpei,Cui Wuren,Li Jianhua,Ye Weixin Department of Nuclear Medicine,Research Center of Experimental Medicine,Tongji Medical University,WuhanFang Weiyi,Wei WenhuaDepartment of Radioimmunoassay,TongjiHospital,Tongji Medical University,Wuhan |
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| Abstract: | An experiment was conducted with ten kinds of RIA Kit to compare separation technique based on solid phase double antibody magnitizable particle (DAMP) with common methods of double antibody, polyethylene glycol (PEG) and charcoal. It was found that the five quality control indices for DAMP method and double anti body method are within the scope of RIA quality control standard, and that DAMP method has the same separating effect as double antibody method. However, the former is simpler and more rapid than the latter. As a result, it is suitabe for universal separation in RIA. |
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| Keywords: | radioimmunoassay separation technique quality control |
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