Molecular characterisation of two novel double-stranded RNA elements from <Emphasis Type="Italic">Phlebiopsis gigantea</Emphasis> |
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Authors: | Zisis Kozlakidis Caroline V Hacker Dominic Bradley Atif Jamal Xiyu Phoon Joan Webber Clive M Brasier Kenneth W Buck and Robert H A Coutts |
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Institution: | (1) Division of Biology, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London, SW7 2AZ, UK;(2) Forest Research Agencies, Alice Holt Lodge, Farnham, Surrey, GU10 4LH, UK |
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Abstract: | The incomplete sequences of two large, 10–12 kbp, double-stranded RNAs (dsRNAs) found in the TW-2 isolate of the saprophytic
fungus, Phlebiopsis gigantea (Pg) are reported. Both PgV-TW2 dsRNA1 and dsRNA2 potentially encode fusion proteins which are apparently expressed by a
translational frameshifting mechanism. The C-terminal region of both predicted proteins was 21% identical and contained the
eight motifs conserved in RNA-dependent RNA polymerases of dsRNA mycoviruses and had highest similarity with members of the
family Totiviridae, but possibly do not form virions. The remainder of the N-terminal protein sequences predicted from the PgV-TW2 dsRNA1 and
dsRNA2 sequences and the 3′-terminal nucleotide sequences of both dsRNAs had no homology with one another or any sequence
in the database suggesting that individually both may be members of novel families of mycoviruses.
The nucleotide sequence data reported in this article has been assigned the accession numbers AM111O96 and AM111097 for PgV-TW2
dsRNA1 and PgV-TW2 dsRNA2 respectively. |
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Keywords: | Mycovirus dsRNA Phlebiopsis gigantea |
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