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| 银杏内酯对过氧化氢损伤的皮层神经元和SH—SY5Y细胞亲环蛋白A（CypA）表达的影响 | |
| 引用本文： | 杨俊霞,杨惠超,王东江,潘志强.银杏内酯对过氧化氢损伤的皮层神经元和SH—SY5Y细胞亲环蛋白A（CypA）表达的影响[J].中国医药导报,2011,8(17):5-8. |
| 作者姓名： | 杨俊霞 杨惠超 王东江 潘志强 |
| 作者单位： | 1. 徐州医学院江苏省麻醉学重点实验室&江苏省麻醉与镇痛应用技术重点实验室,江苏徐州,221000 2. 北京金康驰医药投资有限公司,北京,100142 3. 河北省临漳县人民医院胸外科,河北临漳,056600 |
| 摘 要： | 目的：研究银杏内酯（ginkgolides,Gin）对过氧化氢（H2O2）诱导的皮层神经元及SH-SY5Y细胞（神经母细胞瘤细胞）损伤的保护作用及其机制。方法：在培养1周的小鼠皮层神经元培养基中加入H2O2,建立神经元氧化损伤模型,用同样方法建立SH-SY5Y细胞氧化损伤模型。采用MTT比色法检测细胞活力,Western Blot技术分析亲环蛋白A的表达变化。结果：H2O2可诱导神经元和SH-SY5Y细胞损伤,使细胞活力降低,可下调亲环蛋白A的表达;Gin预处理12 h可提高细胞活力,可使亲环蛋白A表达增加。结论：Gin可抑制H2O2引起的神经元及SH-SY5Y细胞损伤,该保护作用与其上调亲环蛋白A的表达有关。 |
| 关 键 词： | 银杏内酯 过氧化氢 亲环蛋白A 神经元 SH-SY5Y细胞 |
Effect of Ginkgolides on the expression of CypA in primary cultured neurons and SH-SY5Y cells injuried by H2O2 |
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| YANG Junxia,YANG Huichao,WANG Dongjiang,PAN Zhiqiang.Effect of Ginkgolides on the expression of CypA in primary cultured neurons and SH-SY5Y cells injuried by H2O2[J].China Medical Herald,2011,8(17):5-8. | |
| Authors: | YANG Junxia YANG Huichao WANG Dongjiang PAN Zhiqiang |
| Institution: | 1.Jiangsu Province Key Laboratory of Anesthesiology;Jiangsu Province Key Laboratory of Anesthetic and Analgesic Application Technology;Xuzhou Medical College;Xuzhou 221000;2.Beijing King Health Investment Co.Ltd.,Beijing 100142;3.Department of Chest Surgery,Linzhang County People′s Hospital,Linzhang 056600 |
| Abstract: | Objective: To study the protective effects of ginkgolides(Gin) in primary cultured neurons and SH-SY5Y cells injuried by H2O2 and its action mechanism.Methods: Oxidative injury model of neurons and SH-SY5Y cells was induced by H2O2 in mice.The cell activity was detected by MTT,meanwhile the expression of CypA was observed by Western Blot.Results: H2O2 could both induce oxidative injury in neurons and SH-SY5Y cells,decreased the cell activity,inhibited the expression of CypA.Besides ginkgolides preconditioni... |
| Keywords: | Ginkgolides H2O2 CypA Neurons SH-HY5Y cells |
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