Bluetongue virus RNA detection by RT-qPCR in blood samples of sheep vaccinated with a commercially available inactivated BTV-8 vaccine |
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Authors: | Adolf Steinrigl,Sandra Revilla-Ferná ndez,Michaela Eichinger,Josef Koefer,Petra Winter |
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Affiliation: | 1. Austrian Agency for Health and Food Safety (AGES), Institute for Veterinary Disease Control Moedling, Robert Koch-Gasse 17, A-2340 Moedling, Austria;2. Institute for Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Austria |
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Abstract: | In 2008, a country-wide bluetongue virus 8 (BTV-8) vaccination campaign has been initiated in Austria, using a single commercial inactivated BTV-8 vaccine. Based on preliminary data, we hypothesised that vaccine-derived BTV RNA is transiently detectable by reverse transcription quantitative real-time PCR (RT-qPCR) in the blood of vaccinated animals. Thus BTV-8 vaccine was administered to five BTV-naïve adult sheep and blood samples were taken at various time-points post-vaccination. BTV RNA was detectable by several RT-qPCR methods in all five animals, mainly within the first 9 days post-vaccination. These results show that RT-qPCR based testing for BTV-infection may be influenced by vaccination with certain inactivated BTV-8 vaccines. |
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Keywords: | Bluetongue virus RT-qPCR BTV-8 vaccination |
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