Identification and characterization of nematode specific protective epitopes of Brugia malayi TRX towards development of synthetic vaccine construct for lymphatic filariasis |
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Authors: | Jayaprakasam Madhumathi Prabhu Rajaiah Prince Gandhirajan Anugraha Pote Kiran Donthamsetty Nageswara Rao Maryada Venkata Rami Reddy Perumal Kaliraj |
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Affiliation: | 1. Centre for Biotechnology, Anna University, Sardar Patel Road, Chennai 600025, Tamil Nadu, India;2. Department of Biochemistry, All India Institute of Medical Sciences, New Delhi 110029, India;3. Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram 442102, India |
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Abstract: | Although multi-epitope vaccines have been evaluated for various diseases, they have not yet been investigated for lymphatic filariasis. Here, we report for the first time identification of two immunodominant B epitopes (TRXP1 and TRXP2) from the antioxidant Brugia malayi thioredoxin by studying their immune responses in mice model and human subjects. TRXP1 was also found to harbor a T epitope recognized by human PBMCs and mice splenocytes. Further, the epitopic peptides were synthesized as a single peptide conjugate (PC1) and their prophylactic efficacy was tested in a murine model of filariasis with L3 larvae. PC1 conferred a significantly high protection (75.14%) (P < 0.0001) compared to control (3.7%) and recombinant TRX (63.03%) (P < 0.018) in experimental filariasis. Our results suggest that multi-epitope vaccines could be a promising strategy in the control of lymphatic filariasis. |
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Keywords: | Filariasis Multi-epitope vaccine Thioredoxin |
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