Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes |
| |
| Authors: | Laï la-Aï cha Hanafi,Marilè ne Bolduc,Marie-È ve Laliberté Gagné ,Florent Dufour,Yves Langelier,Mohammed-Rachid Boulassel,Jean-Pierre Routy,Denis Leclerc,Ré jean Lapointe |
| |
| Affiliation: | 1. Research Centre, Centre hospitalier de l’Université de Montréal (CRCHUM) – Hôpital Notre-Dame, Université de Montréal and Institut du Cancer de Montréal, Montréal, Québec, Canada;2. Centre de recherche en infectiologie, Pavillon Centre hospitalier de l’Université Laval (CHUL), Université Laval, 2705 boul. Laurier, Québec, Québec, Canada;3. Immunodeficiency Service and Division of Haematology, Royal Victoria Hospital, McGill University Health Center, McGill University, Montréal, Québec, Canada;4. Research Centre, CHUM – Hôpital St-Luc, Montréal, Québec, Canada |
| |
| Abstract: | Chimeric VLPs made of papaya mosaic virus (PapMV) trigger a CTL response through antigenic presentation of epitopes on MHC class I. Here, a chimeric VLP composed of malva mosaic virus (MaMV) was shown to share similar properties. We demonstrated the capacity of both VLPs to enter human APCs. The chimeric constructions were cross-presented in CD40-activated B lymphocytes leading to in vitro expansion of antigen-specific T lymphocytes. We showed that high concentrations of chimeric MaMV induced cell death, suggesting that some modifications can trigger collateral effects in vitro. Results suggest that potexvirus VLPs are an attractive vaccine platform for inducing a CTL response. |
| |
| Keywords: | APC, antigen presenting cells PapMV, papaya mosaic virus MaMV, malva mosaic virus CP, coat protein CD40B, CD40-activated B lymphocytes VLP, virus-like particles CTL, cytotoxic T lymphocytes moDC, monocyte-derived DC |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
