|
|||||
|
|
Lipopolysaccharide from Burkholderia thailandensis E264 provides protection in a murine model of melioidosis |
|
| Authors: | Sarah A. Ngugi Valeria V. Ventura Omar Qazi Sarah V. Harding G. Barrie Kitto D. Mark Estes Anne Dell Richard W. Titball Timothy P. Atkins Katherine A. Brown Paul G. Hitchen Joann L. Prior |
| Affiliation: | 1. Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, UK;2. Department of Life Sciences, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK;3. Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA;4. Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA;5. Department of Microbiology and Immunology, Department of Pathology and the Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555, USA;6. University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK;g Centre for Integrative Systems Biology, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK |
| Abstract: | Burkholderia thailandensis is a less virulent close relative of Burkholderia pseudomallei, a CDC category B biothreat agent. We have previously shown that lipopolysaccharide (LPS) extracted from B. pseudomallei can provide protection against a lethal challenge of B. pseudomallei in a mouse model of melioidosis. Sugar analysis on LPS from B. thailandensis strain E264 confirmed that this polysaccharide has a similar structure to LPS from B. pseudomallei. Mice were immunised with LPS from B. thailandensis or B. pseudomallei and challenged with a lethal dose of B. pseudomallei strain K96243. Similar protection levels were observed when either LPS was used as the immunogen. This data suggests that B. thailandensis LPS has the potential to be used as part of a subunit based vaccine against pathogenic B. pseudomallei. |
| Keywords: | Burkholderia thailandensis Burkholderia pseudomallei Lipopolysaccharide |
| 本文献已被 ScienceDirect 等数据库收录！ | |