脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

目的探讨脑星形细胞瘤中TIP30、血管内皮生长因子（VEGF）、CD34的表达情况及其病理意义。方法采用免疫组织化学Elivision plus两步法，检测19例正常脑组织及71例星形细胞瘤组织中TIP30、VEGF及CD34的表达。结果TIP30在19例正常脑组织神经胶质细胞及神经元胞质内均呈阳性表达；71例星形细胞瘤组织中，TIP30总阳性表达率为33．80％，随星形细胞瘤分化程度的降低，TIP30的表达逐渐降低，Ⅱ、Ⅲ和Ⅳ级星形细胞瘤中TIP30阳性表达率分别为52％、34．78％和13．04％。高级别星形细胞瘤（Ⅲ级＋Ⅳ级）中TIP30的阳性表达率显著低于其在低级别星形细胞瘤（Ⅱ级）中的阳性表达率（x^2=5．71，P〈0．05）；VEGF及由CD34确定的MVD在星形细胞瘤中表达均高于正常脑组织，且随星形细胞瘤病理级别升高表达逐渐增高；星形细胞瘤中TIP30与VEGF强阳性表达呈负相关关系（r=-0．428，P〈0．05）。而TIP30与MVD无明显相关性（r=-0．065，P〉0．05）。结论TIP30在正常脑组织中阳性表达率高于其在星形细胞瘤中阳性表达率，且随星形细胞瘤病理级别升高表达显著下降；星形细胞瘤中，VEGF的表达与TIP30的表达呈明显的负相关关系。

Expression and significance of TIP30, VEGF and CD34 in brain astrocytoma

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.