氯化钆对血吸虫肉芽肿期肝脏调节性T细胞的抑制作用

目的探讨氯化钆封闭Kupffer细胞功能对日本血吸虫病肉芽肿期CD4+CD25+T细胞的影响。方法6～8w龄雌性C57BL/6小鼠分为3组,即对照组、感染组与感染/氯化钆组,其中氯化钆为15 mg/kg,尾静脉注射,每w2次;感染第8w流式细胞仪检测CD4+CD25+T细胞的数量;免疫组织化学染色检测Foxp3的分布;酶联免疫吸附试验检测血细胞因子IL-4、IL-5、IL-10、TGF-β1与IFN-γ的表达水平。结果感染小鼠CD4+CD25+T细胞数量为13.8%,感染/氯化钆组为9.3%(p<0.05),对照组为6.4%;IL-10在感染组为41.4 pg/mL,感染/氯化钆组22.6 pg/mL;此外,氯化钆还能下调Foxp3的分布以及减轻血吸虫肉芽肿周围的炎症反应。结论氯化钆封闭Kupffer细胞的功能后可减少日本血吸虫感染肉芽肿期CD4+CD25+T细胞的产生以及减轻肉芽肿周围的炎症反应。

The inhibitory effect of gadolinium chloride on the regulatory T cells of mice with hepatic granuloma caused by Schistosoma japonicum

To determine whether the targeting inhibition of Kupffer cellsfunction mediated by gadolinium chloride(GdCl3) could interfere with the CD4+CD25+ regulatory T cells of mice at the granuloma stage of schistsomiasis,female C57BL/6 mice of 6-8 weeks old were divided randomly into 3 groups,i.e.control group.group infected with cercariae of Schistsoma japonicum and group of infection plus GdCl3,.GdCl3 in a dosage of 15 mg/kg was introduced into mice through penile vein twice per week.The number of CD4+CD25+ T cells was determined using flow cytometry and the number of cells with Fox p3 was detected by using immunohistochemical methods.For detection of cytokines,such as IL-4,IL-5,IL-10,TGF-β1,,IFN-γ in mouse sera,a DuoSer ELISA development kit was used,It was found that the number of CD4+CD25+ T cells and level of IL-10 in Schistosomiasis granuloma stage were decreased in the S.japonicum cercariae infected mice injected with GdCl3 in comparison with the infection group.The percentages of CD4+CD25+ T cells of infection group and infection plus GdCl3 group were 13.8%,9.3% and 6.4% respectively,while the levels of IL-10 of these 3 groups of rats were 41.4 pg/mL,22.6 pg/mL and 11.5% respectively.In addition,treatment with GdCl3 could down-regulate the expression of Fox p3 and reduce the inflammatory reactions in Schistosomiasis granuloma.It is evident that the targeting inhibition of Kupffer cellsfunction mediated by GdCI3 interfere with the production of the regulatory T cells and reduce the inflammatory responses in Schistsomiasis granuloma.