纳米微囊-VEGF复合体对创伤组织中MMP-9及TIMP-1mRNA表达的影响

目的探讨纳米微囊-VEGF复合体对创伤组织中MMP-9及TIMP-1mRNA表达的影响。方法利用纳米微囊-VEGF复合体作用于兔耳慢性创面,实验分为转基因组(CW+VEGF)、慢性创面组(CW)、非缺血创面组(NW),通过反转录PCR方法依次观察术后1d、3d、7d、10d、14d损伤组织中MMP-9及TIMP-1mRNA表达的变化情况。结果1MMP-9mRNA在CW组3~14d的表达水平高于NW组在相应时间点的表达水平,具有显著差异(P<0.05);CW+VEGF组在10d及14d表达水平显著高于CW组,具有显著性差异(P<0.05)。2TIMP-1mRNA在NW组和CW+VEGF组3~14d的表达水平均高于其在CW组相应时间点的表达水平,具有显著性差异(P<0.05)。结论MMP-9的高表达及TIMP-1的低表达是慢性创面难愈合的重要机制;外源性VEGF基因通过上调TIMP-1的表达促进了慢性创面的愈合。

The effects of complex of nanosphere-VEGF on expression of MMP-9 and TIMP-1mRNA in wounded tissue

Objective: To study the effects of complex of nanosphere-VECF on expression of MMP-9 and TIMP-1mRNA,and investigate its mechanisms of improving wounded tissue repairing. Methods: We excerted complex of nanosphere-VECF on wounded rabit ears, samples were divided into chronic wound+VEGF-transgenic group(CW+VEGF), chronic wound group(CW)and nonischemia wound group(NW),using RT-PCR was used to examine expression of MMP-9 and TIMP-1mRNA on 1d, 3d, 7d, 10d, 14d post operation. Results: ①The expression of MMP-9 mRNA of rabbits in CW was greater than in NW at the same time from 3d to 14d post operation , with statistical significance(P<0.05). ②The expression of TIMP-1 mRNA in NW and CW+VEGF was both greater than in CW at the same time from 3d to 14d post operation, with statistical significance(P<0.05).Conclusion: High expression of MMP-9 and low expression of TIMP-1 might be important mechanisms of chronic raw surface healing; exogenous VEGF gene transfection might promote wound healing through upregulating the expression of TIMP-1.