油松松针中黄酮类成分的分离与鉴定

目的研究油松松针中的黄酮类成分,为松属植物的化学分类学研究提供科学依据。方法采用反复硅胶、聚酰胺、ODS、Sephadex LH-20柱色谱等方法进行分离纯化,根据理化性质和1H-NMR、13C-NMR、质谱等技术对分离得到的化合物进行结构鉴定。结果从油松松针中分离得到4个化合物,分别鉴定为山柰酚-3-O-(3″-O-反式-对-肉桂酰基)-(6″-O-反式-阿魏酰基)-β-D-吡喃葡萄糖苷kaempferol 3-O-(3″-O-E-p-coumaroyl)-(6″-O-E-feruloyl)-β-D-glucopyranoside,1]、山柰酚-3-O-(3″,6″-二-反式-对-肉桂酰基)-β-D-吡喃葡萄糖苷kaempferol3-O-(3″,6″-di-O-E-p-coumaroyl)-β-D-glucopyranoside,2]、山柰酚-3-O-(3″-反式-对-肉桂酰基)-β-D-吡喃葡萄糖苷kaempferol3-O-(3″-O-E-p-coumaroyl)-β-D-glucopyranoside,3]、异鼠李素-3-O-β-D-吡喃葡萄糖苷isorhamnetin3-O-β-D-glucopyranoside,4]。结论化合物1、3为首次从松属植物中分离得到,化合物2、4为首次从该种植物中分离得到。

Isolation and identification of flavonoids from pine needle of Pinus Tabulaeformis Carr.

objective To study the flavonoids of pine needles of Pinus tabulaeformis Carr. Methods Compounds were isolated by the silica gel, polyamide, ODS, Sephadex LH-20 columns, and their structures were identified by the physicochemical characters and 1H-NMR, 13C-NMR, MS. Results Four compounds were as kaempferol 3-O-(3"-O-E-p-coumaroyl)-(6"-O-E-feruloyl)-β-D-glucopyranoside(1), kaempferol 3-O-(3",6"-di-O-E-p-co- umaroyl)-β-D-glucopyranoside(2), kaempferol 3-O-(3′′-O-E-p-coumaroyl)-β-D-glucopyranoside(3), isorhamnetin 3-O-β -D-glucopyr-anoside(4). Conclusions Compounds 1 and 3 are isolated from Pinus for the first time; Compounds 2 and 4 are isolated from this species for the first time.