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不同剂量致敏原对小鼠哮喘模型气道反应性的影响
引用本文:唐晓媛,于化鹏,邓火金,陈新,樊慧珍,龚雨新,刘俊芳.不同剂量致敏原对小鼠哮喘模型气道反应性的影响[J].现代医学,2011,39(2):121-125.
作者姓名:唐晓媛  于化鹏  邓火金  陈新  樊慧珍  龚雨新  刘俊芳
作者单位:南方医科大学珠江医院,呼吸内科,广东,广州,510282
基金项目:广东省自然科学基金资助项目
摘    要:目的:探讨不同剂量致敏原鸡卵蛋白(OVA)制备哮喘模型时对哮喘小鼠气道反应性的影响。方法:将30只BALB/c小鼠随机分为哮喘组和正常对照组(C组),哮喘组又分为小剂量OVA致敏组(A组)和大剂量OVA致敏组(B组),每组各10只。A、B组在开始和第14天时给予含OVA的致敏液200μl(A组含10μg OVA,B组含2 mg OVA,两者含同样的氢氧化铝及生理盐水),21 d开始雾化吸入OVA,连续雾化6 d,操作时观察小鼠活动及呼吸情况;各组分别于末次雾化激发24 h后测定小鼠的无创肺功能中的增强呼气间歇(Penh)值以评价气道反应性;对支气管肺泡灌洗液(BALF)行细胞总数、嗜酸粒细胞计数;取肺组织作HE染色病理切片;ELISA方法测外周血、BALF上清液中的IgE、IFN-γ含量。结果:哮喘B组在第二次致敏后可见喘息、呼吸困难、口唇和尾巴黏膜发紫表现,并在雾化时出现扭体、燥动、呼吸加快等症状。哮喘A、B组的Penh值明显高于C组(P<0.01);从乙酰甲胆碱(Mch)6.25 mg.m l-1开始时哮喘B组的Penh值明显高于哮喘A组(P<0.01)。哮喘A组的BALF中细胞总数、嗜酸粒细胞数(2.41±0.90,0.93±0.18)较正常对照组明显增高(0.65±0.34,0.30±0.16,均P<0.05);哮喘B组中BALF中细胞总数、嗜酸粒细胞数(4.89±3.49,1.74±0.76)较正常对照组增高(均P<0.05);哮喘B组BALF中细胞总数、嗜酸粒细胞数与哮喘A组比较差异无统计学意义。哮喘A、B组的支气管、血管周围,肺间质及肺泡腔内可见嗜酸粒细胞、淋巴细胞浸润,气道上皮损伤,结构紊乱,气道内杯状细胞肥大增生,气道内分泌大量黏液并有黏液栓形成;正常对照组肺组织气道结构清晰,气道纤毛上皮排列整齐,无明显的炎症改变。哮喘A、B组中外周血和BALF的IgE含量均较正常组的增多(P<0.01);哮喘B组中外周血及BALF的IgE含量(37.47±6.18,26.10±7.18)较哮喘A组(26.09±3.76,12.47±3.02,均P<0.01)明显增多。哮喘A、B组中外周血和BALF中的IFN-γ含量均较正常组减少(P<0.01);哮喘B组中外周血及BALF的IFN-γ含量(35.29±10.92,37.44±21.01)与哮喘A组比较差异无统计学意义(32.22±10.04,36.89±22.00,均P>0.05)。结论:哮喘小鼠组模型制备成功。在观察气道高反应方面,较高剂量OVA致敏哮喘模型较低剂量OVA致敏的气道高反应性更敏感。

关 键 词:哮喘小鼠模型  气道反应性  鸡卵蛋白  支气管肺泡灌洗液

Different doeses of ovalbumin sensitization in airway responsiveness in a murine model of asthma
TANG Xiao-yuan,YU Hua-peng,DENG Huo-jin,CHEN Xin,FAN Hui-zhen,GONG Yu-xin,LIU Jun-fang.Different doeses of ovalbumin sensitization in airway responsiveness in a murine model of asthma[J].Modern Medical JOurnal,2011,39(2):121-125.
Authors:TANG Xiao-yuan  YU Hua-peng  DENG Huo-jin  CHEN Xin  FAN Hui-zhen  GONG Yu-xin  LIU Jun-fang
Institution:(Department of Respiratory Medicine,Zhujiang Hospital,Southern Medical University,GuangZhou 510282,China)
Abstract:Objective:To explore the different does of ovalbumin(OVA) sensitization whether have influences on airway hyper responsiveness of mice model of asthma.Methods:The mice were randomly divided into 3 groups,with 10 mice in each group:control group and asthmatic groups(including asthma A group,the does of OVA sensitization was 10 μg every time;and asthma B group,which was 2 mg).The asthmatic A and B group were sensitized on the 0th,14th day with different does of OVA,then inhaled OVA beginning on the 21st day for 6 days.Meanwhile the breathing and the color of oral and tail mucosa were monitered.The last time OVA stimulated and 24 hours later,Penh were measured to evaluate the airway responsiveness by noninvasive lung functional instrument,bronchoalveolar lavage cytology was performed and lung tissue sections were prepared for histopathologic examination to evaluate the airway inflammation.The IgE,IFN-γ of blood and BALF were detected by ELISA method.Results:The skin of mice lip and tail was caynosis after the second allergic sensitization in asthmatic B group mice.The airway hyper responsiveness in asthmatic group was significantly higher than that in the control mice,and asthmatic B group’s Penh was significantly higher than asthmatic A group.The total cell number and the eosinophils in BALF of the asthmatic mice were significantly higher than that in the control mice;The total cell number and the eosinophils in BALF of the asthmatic B group were insignificantly higher than that in asthmatic A group.The contents of IgE in peripheral blood and BALF of the asthmatic mice were significantly higher than that in the control mice,and which in the asthmatic B group were significantly higher than that in asthmatic A group.The contents of IFN-γ in peripheral blood and BALF of the asthmatic mice were significantly lower than that in control ones,were insignificantly higher in the asthmatic B group than those in asthmatic A group.Conclusion:A murine model of asthma is successfully established.The one sensitized with high does of OVA tends to have higher responsiveness than the group with low inducing does of OVA.
Keywords:mice model of asthmac  ovalbumin  airway responsiveness  BALF
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