丙基硫氧嘧啶不同摄入方式对大鼠动脉粥样硬化形成的影响

目的:比较2种不同的建模方法,探索建立成模率高且重复性好的大鼠动脉粥样硬化模型的最佳方案。方法:将30只健康雄性W istar大鼠随机分为空白对照组、A组和B组。空白对照组使用基础饲料喂养。A组:维生素D3腹腔注射+高脂饲料(含丙基硫氧嘧啶)进食。B组:维生素D3腹腔注射+高脂饲料(不含丙基硫氧嘧啶)+丙基硫氧嘧啶灌胃。建模时间90 d,处死大鼠后检测血脂水平,动脉内膜行油红O染色及制作石蜡切片、HE染色形态学观察。结果:空白对照组、B组动物均无死亡,A组死亡4只。B组动物血清总胆固醇(TC)、低密度脂蛋白(LDL)水平明显高于A组(均P<0.01)。油红O染色显示B组阳性病灶最多,A组较少,空白对照组偶见。光镜下空白对照组大鼠未见动脉粥样硬化病变,A组大鼠出现动脉粥样硬化初期改变,B组大鼠出现典型的动脉粥样硬化斑块或钙化灶。结论:维生素D3+高脂饲料+丙基硫氧嘧啶灌胃可建立典型的动脉粥样硬化模型,成模率高,死亡率低。

Effect of propylthiouracil administrated in different way on the formation of atherosclerosis in rats

Objective:To investigate a simple and successful method for establishing atherosclerotic rat model.Methods:Thirty healthy male Wistar rats were randomly divided into three groups:control group(n=10);model group A(n=10):treated by 6×105 unit vitamin D and high-fat diet(containing propylthiouracil) for 90 days;model group B(n=10):treated by 6×105 unit vitamin D and high-fat diet(not containing propylthiouracil and fed by intragastric administration) for 90 days.Serum lipid and morphological change of aorta were determined after sacrifice.Results:There were 4 rats in group A dead while no rats died in the other two groups.The levels of serum total cholesterol(TC) and low density lipoprotein(LDL) in model group B were significantly higher than those in model group A(each P<0.01).Staining artery by rathonum red showed that there were massive positive niduses in group B while less in group A,and sporadic in the control group.The control group almost had no morphologic changes under light microscope,while rats existed inchoate change of atherosclerosis in model group A,and rats existed typical atherosclerosis in model group B.Conclusion:Method of 6×105 unit vitamin D intraperitoneal injection,high-fat diet not containing propylthiouracil which was feed by intragastric administration is convenient and stable to establish typical atherosclerotic rat model.