pSS患者唇腺组织中Fas介导的凋亡信号蛋白的表达

目的:研究原发性干燥综合症( primary Sjgren’s syndrome，pSS)唇腺组织细胞中Fas 介导的细胞凋亡信号蛋白的表达，探讨导致干燥综合征的细胞信号转导的可能途径。 方法:采用Western blotting法检测唇腺组织中Fas、FasL、FADD蛋白的表达;免疫组织化学法检测caspase-3蛋白表达;RT-PCR半定量检测CAD mRNA表达。 结果:pSS患者唇腺组织细胞中Fas 介导的细胞凋亡信号转导相关蛋白Fas、FasL、FADD、caspase-3及CAD mRNA表达都有不同程度高于对照组(P＜0.05)。 结论:导致干燥综合征患者唇腺组织凋亡的信号转导途径可能是：FasL与其受体Fas结合后，通过FADD激活caspase-8，级联激活ICE家族成员，最后通过caspase-3裂解ICAD释放CAD催化DNA降解导致细胞凋亡。

Expression of apoptosis signal protein induced by Fas in labial salivary gland of patients with primary Sj(ǒ)gren's syndrome

AIM:To study the expression of apoptosis signal proteins induced by Fas in labial salivary gland of patients with primary Sjgren’s syndrome (pSS), and investigate the possible pathway of the cell signaling transduction in pSS. METHODS:Biopsies of minor submucosal labial salivary gland were obtained from 32 patients with pSS and 8 normal controls. Fas, FasL and FADD were detected by Western blotting. Caspase-3 was measured by immunohistochemistry and CAD mRNA expression was determined by RT-PCR. RESULTS:The expressions of Fas, FasL, FADD, caspase-3 and CAD mRNA in labial salivary glands of patients with pSS were significantly higher than those in control group (P＜0.05). CONCLUSION:The apoptosis signalling transduction in labial salivary glands of patients with pSS may be that the product of FasL combined with Fas activates caspase-8 through FADD, then ICE family is cascaded, and finally CAD is splited by caspase-3 from ICAD, which catalyzes the degradation of DNA. 