| 优选超临界CO2萃取灵芝孢子粉中总三萜化合物的最佳工艺 |
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| 引用本文： | 陈燕,柳正良,陈志东,陆瑶华,郭澄. 优选超临界CO2萃取灵芝孢子粉中总三萜化合物的最佳工艺[J]. 药学服务与研究, 2010, 10(1): 34-36 |
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| 作者姓名： | 陈燕 柳正良 陈志东 陆瑶华 郭澄 |
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| 作者单位： | 1. 上海交通大学附属第六人民医院药剂科,上海,200233
2. 第二军医大学药学院药物分析学教研室,上海,200433 |
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| 摘 要： | 目的：建立超临界CO2萃取法提取和纯化灵芝孢子粉中总三萜化合物的最佳工艺条件。方法：采用超临界CO2萃取技术,以正交试验考察提取压力、温度和时间对提取效果的影响。结果：最佳工艺条件为萃取压力22 MPa,萃取温度50℃,萃取时间2 h。总三萜化合物的回收率为17.6%,在灵芝孢子粉中含量为160 mg/g。结论：优选出的工艺稳定可靠,简便易行。
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| 关 键 词： | 灵芝孢子粉 超临界CO2萃取 总三萜化合物 提取工艺 正交试验 |
Optimal technology for extraction of total triterpenoid compounds from Ganoderma lucidum spores by supercritical CO_2 extraction |
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| CHEN Yan,LIU Zhengliang,CHEN Zhidong,LU Yaohua,GUO Cheng. Optimal technology for extraction of total triterpenoid compounds from Ganoderma lucidum spores by supercritical CO_2 extraction[J]. Pharmaceutical Care and Research, 2010, 10(1): 34-36 |
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| Authors: | CHEN Yan LIU Zhengliang CHEN Zhidong LU Yaohua GUO Cheng |
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| Affiliation: | 1.Department of Pharmacy,the Sixth People＇s Hospital affiliated to Shanghai Jiaotong University,Shanghai 200233,China;2.Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433,China) |
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| Abstract: | Objective:To establish the optimal technology for extraction and purification of total triterpenoids from Ganoderma lucidum spores by supercritical CO_2 extraction.Methods:The influence of different factors such as extraction pressure,temperature and time on the extraction efficiency was studied. The technique of supercritical CO_2 extraction was optimized by orthogonal tests.Results:The optimal extraction technique was as follows:extraction pressure was 22 MPa,extraction temperature was 50 ℃,and extraction time was 2 h.The extraction efficiency of total triterpenoids compounds was 17.6% and the content of total triterpenoids in Ganoderma lucidum spores was 160 mg/g.Conclusion:The optimal technology is stable,reliable, convenient and practical for extraction of total triterpenoids from Ganoderma lucidum. |
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| Keywords: | Ganoderma lucidum spore supercritical CO_2 extraction total triterpenoids extraction technique orthogonal test |
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