静电纺丝薄膜支架对MC3T3-E1细胞增殖分化的影响

目的 观察静电纺丝薄膜支架对MC3T3-E1细胞增殖分化的影响。方法 通过静电纺丝法制备薄膜支架,扫描电镜观察薄膜网状结构,通过薄膜支架作为载体,观察其对成骨细胞的增殖、分化并观察细胞骨架的形态变化。结果 扫描电镜显示:静电纺丝薄膜网状结构有利于细胞的黏附;CCK-8细胞增殖检测与碱性磷酸酶活性检测结果显示,培养1 d、4 d、7 d对照组与实验组相比差异均有统计学意义(P<0.05);细胞骨架染色显示静电纺丝组细胞铺展面积增大且数目增多。结论 静电纺丝薄膜支架对成骨细胞增殖、分化以及细胞黏附具有促进作用。

Effect of electrospinning film stent on proliferation and differentiation of MC3T3-E1 cells

Objective To observe the effect of electrospinning membrane scaffold on proliferation and differentiation of MC3T3-E1 cells. Methods The membrane scaffolds were prepared by electrospinning. The reticular network was observed by scanning electron microscopy. The membrane scaffolds were used as carriers to observe the proliferation and differentiation of osteoblasts and observe the morphological changes of cytoskeleton. Results Scanning electron microscopy showed that the electrospun membrane network structure was conducive to cell adhesion; CCK-8 cell proliferation assay and alkaline phosphatase activity persisted to the results, and the difference between the control group and the experimental group was 1 d, 4 d and 7 d. All of them were statistically significant (P<0.05); cytoskeletal staining showed that the cell spreading area of the electrospinning group increased and the number increased. Conclusion Electrospun membrane stents can promote osteoblast proliferation, differentiation and cell adhesion.