自然排卵、促排卵和超促排卵状态下小鼠卵巢组织中生长分化因子-9(GDF-9)的表达

目的:观察自然周期排卵、促排卵与控制性超促排卵(COH)状态下小鼠卵巢生长分化因子-9(growth differentiation factor,GDF-9)表达水平的变化情况。方法:成年雌性小鼠随机分为3组每组10只,分别为自然排卵组(对照组)、孕马血清(PMSG)促排卵组(PMSG组)、控制性超促排卵组(COH组),各组小鼠取排卵期卵巢组织,采用免疫组织化学方法检测GDF-9的表达与定位,并分别采用Western blottting和实时荧光定量PCR法,定量检测GDF-9蛋白与mRNA的含量。结果:各组小鼠卵母细胞与颗粒细胞中均检测到GDF-9的表达,PMSG组与COH组GDF-9蛋白与mRNA的表达水平均显著高于对照组(P<0.05),PMSG组的表达也明显弱于COH组(P<0.05)。结论:促排卵可促进小鼠卵巢GDF-9的表达,但COH降低促排卵小鼠卵巢GDF-9的表达。

Expression of Grow Differentiation Factor 9 (GDF-9) in Mouse Ovaries under Natural Ovulation, Induced Ovulation or Controlled Ovarian Hyper-stimulation

Objective: To investigate the expression of GDF-9 in mouse ovaries under natural ovulation,induced ovulation or controlled ovarian hyperstimulation(COH),respectively.Methods: Adult female mice were divided into three groups: natural ovulation group(the control),PMSG induced ovulation group(PMSG group) and controlled ovarian hyperstimulation group(COH group),10 mice per group.Ovary tissues were collected on ovulation period,and the expression and the location of GDF-9 were detected by immunohistochemistry method.The GDF9 protein was quantitated by Western blotting analysis and GDF-9 mRNA was quantitated by real-time fluorescence quantitative PCR.Results: The expression of GDF-9 was detected both in oocytes and granulosa cells by immunohistochemistry in all the three groups.In PMSG group and COH group,the expression of GDF-9 protein and mRNA levels were dramatically increased compared with the control(P<0.05).While the expression of GDF-9 was significantly down-regulated in COH mice compared with PMSG mice both in protein and mRNA levels(P<0.05).Conclusion: The expression of GDF-9 in mouse ovary was increased by induced ovulation,but the COH procedure deceased the GDF-9 levels compared with the induced ovulation.