| 流式细胞术在外用避孕药精子功能评估中的应用 |
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| 引用本文： | 温成丽,夏敏杰,王玉柱,李卫华.流式细胞术在外用避孕药精子功能评估中的应用[J].生殖与避孕,2013,33(1):48-54. |
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| 作者姓名： | 温成丽 夏敏杰 王玉柱 李卫华 |
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| 作者单位： | 1. 复旦大学公共卫生学院,上海,200032;国家人口计生委计划生育药具重点实验室,上海市计划生育科学研究所,上海,200032
2. 国家人口计生委计划生育药具重点实验室,上海市计划生育科学研究所,上海,200032 |
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| 基金项目： | 上海市人口计生委项目(编号2012JG03);国家自然科学基金项目(编号81100459);上海市科学技术委员会项目(编号11140902900,11PJ1408400) |
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| 摘 要： | 流式细胞术(flow cytometry,FCM)是一种单细胞快速定量分析技术,为精子功能研究提供了一种高通量、多参数的检测手段。FCM在外用避孕药对精子功能评估中的应用,主要包括精子质膜完整性、DNA完整性、顶体反应、线粒体功能、氧化应激和精子凋亡等方面。表明FCM是精子功能研究的有效工具,有助于阐明新型外用避孕药的药效和作用机制。
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| 关 键 词： | 流式细胞术(FCM) 外用避孕药 精子功能 评价 |
Application of Flow Cytometry in Evaluating Spermicidal Function of Topical Contraceptive |
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| Cheng-li WEN,Min-jie XIA,Yu-zhu WANG,Wei-hua LI.Application of Flow Cytometry in Evaluating Spermicidal Function of Topical Contraceptive[J].Reproduction and Contraception,2013,33(1):48-54. |
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| Authors: | Cheng-li WEN Min-jie XIA Yu-zhu WANG Wei-hua LI |
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| Institution: | 1.Fudan University School of Public Health,Shanghai,200032)(2.NPFPC Key Laboratory of Contraceptives and Devices,Shanghai Institute of Planned Parenthood Research,Shanghai,20032) |
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| Abstract: | Flow cytometry(FCM) is a quantitative technology employed in cell counting,sorting,biomarker detection and protein engineering.It allows simultaneous multiparametric analysis of the sperm function.In this review we introduced the application of FCM in the evaluation of topical contraceptives,including plasma membrane integrity,DNA intactness,changes induced during capacitation,mitochondrial status,oxidative stress as well as apoptotic-like changes.It is concluded that FCM is an important tool in the research of sperm function,which helps to clarify the effect and mechanism of novel topical contraceptives. |
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| Keywords: | flow cytometry(FCM) topical contraceptives spermicidal function evaluation |
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