口腔鳞癌中Maspin和VEGF基因的表达及意义

目的：研究乳腺丝氨酸蛋白酶抑制剂基因（marray serine proteinase inhibitor，Maspin）、血管内皮生长因子（vascular endothelial growth factor，VEGF）在口腔鳞癌中的表达及其相关性。方法：应用RT—PCR方法，分别对18例口腔鳞癌的癌组织、癌旁组织及相应正常组织标本行Maspin和VEGF mRNA含量的半定量测定，应用随机区组设计的F检验和Person直线相关分析进行统计分析。结果：Maspin mRNA在口腔鳞癌的癌组织、癌旁组织和正常组织中表达逐渐增加（P〈0．01），且三者之间表达差异均有显著性（P〈0．05）；VEGF mRNA在癌组织、癌旁组织、正常组织中的表达依次降低（P〈0．01），且癌组织和正常组织、癌组织和癌旁组织之间表达差异有显著性（P〈0．05）；口腔鳞癌中，Maspin和VEGF mRNA表达呈负相关（P〈0．01）；Maspin和VEGF基因mRNA表达与肿瘤淋巴结转移相关（P〈0．05），与肿瘤的临床分期和病理分级无关。结论：Maspin基因在口腔鳞癌的发生发展过程中可能起着重要抑癌作用：Maspin可能通过下调VEGF来发挥抑癌作用。

The expression of Maspin and VEGF gene in oral squamous cell carcinoma and its significance

PURPOSE: To study the expression of Maspin(marray serine proteinase inhibitor) and VEGF(vascular endothelial growth factor) gene in oral squamous cell cancers(OSCC) and the relationship between Maspin and VEGF. METHODS: mRNA expression of Maspin and VEGF gene was investigated by RT-PCR in every samples of 18 OSCC cases. F test and Person test were used for data analysis. RESULTS: mRNA expression of Maspin gene increased gradually in OSCC, paratumor and normal epithelium(P<0.01); mRNA expression of VEGF gene decreased gradually in OSCC,paratumor and normal epithelium(P<0.01); The expression of Maspin and VEGF gene was negatively correlated in OSCC(P<0.01) ; The expression of Maspin gene was negatively associated but VEGF gene was positively associated with lymph node metastasis in OSCC(P<0.05); The expression of Maspin and VEGF gene had no relation with the histological grade or tumor size in OSCC. CONCLUSION: Maspin and VEGF gene may play an important role in the development of OSCC and Maspin gene may down-regulate the expression of VEGF gene. Supported by Natural Science Foundation of Hunan Province (Grant No.03JJY3045).