人结肠癌相关成纤维细胞对结肠癌Lovo细胞生物学行为的影响

目的探讨人结肠癌相关成纤维细胞（CAF）对结肠癌Lovo细胞增殖、黏附、迁移和侵袭等生物学行为的影响。方法收集人结肠CAF及正常成纤维细胞（NF）条件培养基，建立结肠CAF、NF与Lovo细胞间的共培养模型，以无血清培养基处理的Lovo细胞作为空白组．进行细胞增殖、黏附、迁移及Transwell侵袭能力检测。结果Lovo细胞与结肠CAF共培养后。增殖48和72h反映细胞增殖的吸光度（A）值分别为（0．667±0．059）和（0．709±0．030），明显高于空白组（0．574±0．031）和（0．593±0．031）]和NF组（0．597±0．036）和（0．652±0．029）]，差异均有统计学意义（P〈0．01）；黏附细胞的A值为（0．588±0．067），也明显高于空白组（0．226±0．039）和NF组（O．375±0．059），差异也均有统计学意义（P〈O．01）；12h和24h细胞迁移率分别为（35．2±8．7）％和（64．6±7．1）％，明显高于空白组（14．7±3．6）％和（26．3±10．3）％]和NF组（14．1±7．3）％和（35．2±9．8）％]（P〈O．01）；透膜细胞数为（56．2±4．8）个，多于空白组的（14．6±2．2）个和NF组的（22．9~4．5）个（P〈O．01）。结论人结肠CAF具有增强结肠癌Lovo细胞增殖、黏附、迁移和侵袭的能力。

Effect of human colon carcinoma-associated fibroblasts on biological behavior of colon carcinoma Lovo cells

Objective To investigate the effects of human colon carcinoma-associated fibroblasts（CAFs） on proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells. Methods The co-culture models among colon CAFs, NFs and Lovo cell were established by conditioned medium（CM）of human colon CAFs and colon normal fibroblasts（NFs）. Lovo cells in the blank control group was treated with serum-free culture medium. The effects of human colon CAFs on proliferation, adhesion, migration and invasion of colon carcinoma Lovo ceils were detected by cell proliferation assay, adhesion assay, migration assay and Transwell invasion assay. Results After coculture with colon CAFs, the absorbance （A） value of Lovo cells was （0.667±0.059） in 48 h and （0.709±0.030） in 72 h. The A value of Lovo cells adhesion to fibronectin was （0.588±0.067）. The cell mobility rates were （35.2±8.7）% in 12 h and （64.6±7.1）% in 24 h. The number of invasive cell was （56.2±4.8）. All the above parameters were increased compared with those in the blank control group and NFs group（all P〈0.01）. Conclusion Human colon CAFs can promote the proliferation, adhesion,migration and invasion of colon carcinoma Lovo cells.