Actin filaments around endothelial fenestrae in rat hepatic sinusoidal endothelial cells |
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| Authors: | Toshihiro Nagai Hiroaki Yokomori Kazunori Yoshimura Kayo Fujimaki Masahiko Nomura Toshifumi Hibi and Masaya Oda |
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| Institution: | (1) Electron Microscopy Laboratory, Keio University, Tokyo, Japan;(2) Department of Internal Medicine, Kitasato Medical Center Hospital, 121-1 Arai, Kitamotoshi, Saitama 364-8501, Japan;(3) Department of Physiology, Saitama Medical School, Saitama, Japan;(4) Department of Internal Medicine, Keio University, Tokyo, Japan;(5) Organized Center of Clinical Medicine, International University of Health and Welfare, Tokyo, Japan |
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| Abstract: | The presence of microfilaments in the vicinity of sinusoidal endothelial fenestrae (SEF) suggests that the cytoskeleton of liver sinusoidal endothelial cells (LSEC) plays an important role in the modulation of SEF. In this study, we investigated actin filaments around SEF in LSECs. Monolayers of LSEC culture were established by infusing a rat liver with collagenase for 30 min and then culturing in RMPI medium for 24h. Cells were reacted with 0.1% Triton X for 5s and 15% glycerinated PHEM buffer (60mM PIPES, 25mM HEPES, 10mM EGTA, 2mM MgCl, pH 6.9) containing heavy meromyosin for 10min and observed under a transmission electron microscope. By electron microscopy with the modified heavy meromyosin decorated reaction, actin filaments were clearly demonstrated around SEF in LSEC. |
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| Keywords: | Heavy meromyosin Actin Hepatic sinusoidal endothelial cell Sinusoidal endothelial fenestrae |
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