| RhoC基因转染对胆管癌QBC939细胞生物学行为的影响 |
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| 引用本文： | 陈明流,石铮,何庆良. RhoC基因转染对胆管癌QBC939细胞生物学行为的影响[J]. 福建医药杂志, 2009, 31(1): 1-4 |
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| 作者姓名： | 陈明流 石铮 何庆良 |
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| 作者单位： | 福建医科大学附属第一医院普外科,350005 |
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| 基金项目： | 福建省自然科学基金资助项目(Z0516021) |
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| 摘 要： | 目的将人RhoC正义、反义基因真核表达载体转染胆管癌QBC939细胞株，研究RhoC基因对胆管癌QBC939细胞株增殖和侵袭能力的影响。方法以脂质体将包含正义、反义RhoC cDNA真核表达载体，分别转染人胆管癌QBC939细胞。应用克隆形成试验检测细胞增殖活性的变化，流式细胞仪检测转染前后细胞周期的变化，Boyden小室侵袭实验检测侵袭及运动能力的变化。结果正义RhoC基因能促进胆管癌QBC939细胞增殖，流式细胞仪分析结果显示，转染后细胞出现G1期细胞减少；侵袭实验显示，转染后细胞侵袭能力较转染前有显著加强。反义RhoC基因能抑制胆管癌QBC939细胞增殖，流式细胞仪分析结果显示，转染后细胞出现G1期细胞增加；侵袭实验显示，转染后细胞侵袭能力较转染前有显著减弱。结论RhoC基因能够促进胆管癌QBC939细胞株的体外增殖和侵袭能力，反义RhoC基因可抑制胆管癌QBC939细胞的增殖和侵袭能力。
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| 关 键 词： | RhoC基因 转染 胆管癌 侵袭 |
Effect of RhoC gene on the biological behaviors of human QBC939 cholangiocarcinoma cell |
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| Chen Mingliu,Shi Zheng,He Qingliang. Effect of RhoC gene on the biological behaviors of human QBC939 cholangiocarcinoma cell[J]. Fujian Medical Journal, 2009, 31(1): 1-4 |
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| Authors: | Chen Mingliu Shi Zheng He Qingliang |
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| Affiliation: | Chen Mingliu,Shi Zheng,He Qingliang.Department of General Surgery,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China |
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| Abstract: | Objective To construct sense and antisense expression vector of human RhoC and observe the effect of RhoC gene on the proliferation and invasion abilities of human cholangiocarcinoma cells.Methods Mammalian expression vector containing RhoC cDNA or antisense RhoC cDNA was transfected into human cholangiocarcinoma cell line(QBC939) with Lipofectane.The cell growth curve was made to determine the inhibitory rate of cells.Flow cytometry was used to analyze the changes of cell cycle of the tumor cells.Boyden ch... |
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| Keywords: | Gene RhoC Transfection Cholangiocarcinoma Invasion |
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