| 基于UPLC-MS/MS代谢组学技术分析不同栽培模式下铁皮石斛类黄酮化合物差异性 |
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| 引用本文： | 李帆,金传高,任仙樱,费璇,赵祺,潘晓军,吴志刚,姜程曦.基于UPLC-MS/MS代谢组学技术分析不同栽培模式下铁皮石斛类黄酮化合物差异性[J].中草药,2022,53(4):1156-1162. |
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| 作者姓名： | 李帆 金传高 任仙樱 费璇 赵祺 潘晓军 吴志刚 姜程曦 |
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| 作者单位： | 温州大学生命科学研究院, 浙江 温州 325035;温州医科大学药学院, 浙江 温州 325035;浙江高鼻子生物科技有限公司, 浙江 乐清 325615;温州医科大学药学院, 浙江 温州 325035;安徽省九华山佛教医药研究所, 安徽 池州 247100;温州大学生命科学研究院, 浙江 温州 325035;安徽省九华山佛教医药研究所, 安徽 池州 247100 |
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| 基金项目： | 温州医科大学人才项目（89220027）；浙江省重点实验室项目（2021E10013）；安徽济人药业有限公司课题（KJHX2009）；合肥市未来药物开发有限公司（KJHX2008）；云南大理药业股份有限公司课题（KJHX1603） |
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| 摘 要： | 目的 研究不同栽培模式下铁皮石斛Dendrobium officinale中类黄酮化合物积累的差异,为铁皮石斛种植方式创新提供依据.方法 运用超高效液相色谱串联质谱(UPLC-MS/MS)广泛靶向技术对3种栽培模式(大棚栽培、林下活树附生和岩壁附生栽培)下的铁皮石斛类黄酮物质进行检测,使用主成分分析(principal...
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| 关 键 词： | 铁皮石斛 UPLC-MS/MS 类黄酮 代谢组学 栽培模式 |
| 收稿时间： | 2021/9/6 0:00:00 |
UPLC-MS/MS metabonomics technology revealing difference of flavonoids in Dendrobium officinale under different planting modes |
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| LI Fan,JIN Chuan-gao,REN Xian-ying,FEI Xuan,ZHAO Qi,PAN Xiao-jun,WU Zhi-gang,JIANG Cheng-xi.UPLC-MS/MS metabonomics technology revealing difference of flavonoids in Dendrobium officinale under different planting modes[J].Chinese Traditional and Herbal Drugs,2022,53(4):1156-1162. |
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| Authors: | LI Fan JIN Chuan-gao REN Xian-ying FEI Xuan ZHAO Qi PAN Xiao-jun WU Zhi-gang JIANG Cheng-xi |
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| Institution: | Institute of Life Sciences, Wenzhou University, Wenzhou 325035, China;School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China;Zhejiang Gaobizi Biological Technology Co., Ltd., Leqing 325615, China;School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China;Jiuhuashan Buddhist Medicine Research Institute, Chizhou 247100, China; Institute of Life Sciences, Wenzhou University, Wenzhou 325035, China;Jiuhuashan Buddhist Medicine Research Institute, Chizhou 247100, China |
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| Abstract: | Objective To reveal the difference of flavonoid components in Dendrobium officinale under different planting modes, in order to provide a basis for the innovation of planting mode. Methods The extensive target metabonomics tool via UPLC-MS/MS workflow was used to detect the flavonoids in D. officinale stem samples produced from three planting modes (including greenhouse, living tree epiphysis planting, and cliff epiphytic planting). Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were performed to identify differential metabolites. Results A total of 202 compounds were identified, mainly including flavonoids (76) and flavonols (60). A novel anthocyanin, cyanidin-3-O- glucoside-7,3''-di-O-(6''-O-Si-pylglucoside) was first detected in D. officinale stem samples. OPLS-DA identified 43 differential compounds, among which 23 flavonoids were found between greenhouse planting and living tree epiphysis planting mode, as well as greenhouse planting and cliff epiphytic planting mode. Additionally, there were 27 differential flavonoids between living tree epiphysis planting and cliff epiphytic planting modes. Overall, the difference of samples from different plant modes was well explained by these differential flavonoids. Conclusion The living tree epiphysis and cliff epiphytic planting modes significantly unregulated the flavonoids biosynthetic pathway relative to greenhouse planting, thus improving the quality of medicinal materials. This result provides a scientific basis for the promotion of novel planting modes within D. officinale production. |
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| Keywords: | Dendrobium officinale Kimura et Migo UPLC-MS/MS flavonoids metabonomics planting mode |
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