双波长HPLC法测定康乐鼻炎片中穿心莲内酯和脱水穿心莲内酯的含量

目的:建立双波长HPLC法测定康乐鼻炎片中穿心莲内酯和脱水穿心莲内酯的含量.方法:采用Kro-masil C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-水(46:54)作为流动相,流速0.7 mL·min-1,柱温35℃,穿心莲内酯检测波长为225 nm,脱水穿心莲内酯检测波长为254 nm.结果:穿心莲...

Determination of andrographolide and dehyandrographolide in Kangle Biyan Tablets by HPLC with dual-wavelength detection

Objective:To establish an HPLC with dual-wavelength detection for the determination of andrographolide and dehyandrographolide in Kangle Biyan Tablets by HPLC.Methods:The determination was performed on Kromasil C₁₈(4.6mm×250 mm,5μm)under the isocratic elution of methanol and water(46∶54).The flow rate was 0.7 mL·min^-1and the column temperature was 35℃.The detection wavelengths of andrographolide and dehyandrographolide were 225 nm and 254 nm respectively.Results:The linear ranges of andrographolide and dehyandrographolide fell into 0.005-0.549μg(r=0.9999)and 0.006-0.560μg(r=0.9999)respectively.The average recoveries of andrographolide and dehyandrographolide were 99.03%with RSD as 0.5%(n=6)and99.03%with RSD as 0.6%(n=6)respectively.Conclusion:The established method was simple,specific,accurate and reliable,and has the good repeatability.It is suitable for the quality control of andrographis paniculata in Kangle Biyan Tablets.