高糖对人腹膜间皮细胞纤维连接蛋白及纤溶酶原激活物抑制剂-1的影响

目的 研究高糖对人腹膜间皮细胞（HPMC）纤维连接蛋白（FN）及纤溶酶原激活物抑制剂-1（PAI-1）的影响。方法 利用HPMC体外培养模型，HPMC分别经含1．5％、2．5％、4．25％葡萄糖的1640培养基培养6、12、24、48h后，用ELISA法检测HPMC分泌FN及PAI-1的情况。结果 ①1．5％、2．5％、4．25％葡萄糖作用于HPMC，使培养上清中的FN明显高于对照组（P〈0．05），且FN增高的程度与葡萄糖浓度和作用时间呈依赖关系。②2．5％、4．25％的葡萄糖作用于HPMC，使培养上清中PAI-1明显高于对照组（P〈0．05），且增高的程度与葡萄糖浓度和作用时间呈依赖关系，1．5％葡萄糖液与对照相比没有差别（P〉0．05）。结论 高糖促使HPMC分泌FN、PAI-1增加，在腹膜纤维化的进程中起一定作用。

Effect of High Glucose on the Expression of Fibronectin and Plasminogen Activator Inhibitor-1 in Human Peritoneal Mesothelial Cells

Objective To evaluate the effect of high glucose on the expression of fibronectin(FN) and plasminogen activator inhibitor-1 (PAI-1) in human peritoneal mesothelial cells(HPMC). Methods The cultured HPMC were exposed to the culture medium containing different concentrations of glucose (1.5%,2.5%,4.25%) for 6,12,24,48 hours, and normal culture medium was used as control. FN and PAI-1 in supernatant were detected by enzyme-linked immunoassay(ELISA). Results The glucose at 1.5%,2.5%,4.25% resulted in a time-and concentration-dependent increase in FN expression in HPMC(P<0.05). The glucose at 2.5%,4.25% increased PAI-1 expression in HPMC(P<0.05), and the effect was time-and concentration-dependent. PAI-1 expression was not affected by 1.5% glucose(P>0.05). Conclusion High glucose increases the expression of FN and PAI-1 in HPMC, thus playing a part in the progression of peritoneal fibrosis.