血链球菌ATCC10556中保守蛋白基因的克隆与分析

目的 克隆并分析血链球菌ATCC10556菌株中一种保守蛋白完整基因。方法 利用Southern印迹杂交分析法、分子克隆及斑点杂交技术获得含有特异片段的目的克隆，对该目的克隆测序，并利用计算机软件和生物信息学技术对克隆的基因及编码蛋白进行分析。结果 首次发现并克隆了血链球菌ATCC10556菌株中完整的保守蛋白基因。将其命名为Sscp基因，该基因在Genbank的登录号：AY032739，该基因编码一种跨膜蛋白，含有两个AAA结构域，结论 Sscp基因编码蛋白的功能与大肠杆菌FtsK相似。参与血链球菌细胞分裂活动。

Cloning and analyzing of the conserved protein gene in Streptococcus sanguinis ATCC10556

Objective To clone and analyze the conserved protein gene in Streptococcus sanguinis(S.s) ATCC10556. Methods Southern blotting hybridization, molecular cloning techniques and dot blotting hybridization were employed to obtain the target clone which harbored the specific DNA fragment.The nucleotide sequence of the target clone was determined. The gene and its product were analyzed by Genework software and bioinformatics techniques. Results The conserved protein gene in Streptococcus sanguinis ATCC10556 was cloned for the first time and named as Streptococcus sanguinis conserved protein gene (Sscp gene). The Genbank accession number for Sscp gene is AY032739.The conserved protein gene in S.s ATCC10556 (named sscp163) was a transmembrane protein and contained two AAA domains.Conclusion sscp163 protein might take part in the cell division in Streptococcus sanguinis; its function is similar to that of FtsK in E.coli.