HPLC法测定氨酚咖黄烷胺片中对乙酰氨基酚和咖啡因的含量

目的建立氨酚咖黄烷胺片中对乙酰氨基酚和咖啡因的含量测定方法。方法采用Hypersil BDS C18色谱柱（150 mm×4.6 mm,5μm）,以甲醇-水（30∶70）为流动相,流速为1.0 ml·min^-1,检测波长为270 nm。结果对乙酰氨基酚在120.06～280.13 mg·L^-1范围内线性关系良好,r=0.999 8,平均回收率为99.89%,RSD=0.23%;无水咖啡因在7.24～16.89 mg·L^-1范围内线性关系良好,r=0.9997,平均回收率为99.70%,RSD=0.50%。结论本法操作简便、准确、快捷,可用于该产品的质量控制。

Determination of paracetamol and caffeine in paracetamol,caffeine,artificial cow-bezoar and amantadine hydrochloride tablets by HPLC

Aim To establish an HPLC method for the determination of paracetamol and caffeine in paracetamol,caffeine,artificial cow-bezoar and amantadine hydrochloride tablets.Methods The Hypersil BDS C18 column was used with methanol-water（30∶70） as a mobile phase.The flow-rate was 1.0 ml·min^-1,and the detection wavelength was at 270 nm.Results There was a good linear relationship for paracetamol（r=0.999 8）at the range of 120.06～280.13 mg·L^-1.The recovery rate was 99.89%,and RSD was 0.23%.And there was a good linear relationship for caffeine （r=0.999 7） at the range of 7.24～16.89 mg·L^-1.The recovery was 99.70%,and RSD was 0.50%.Conclusion The method is convenient,rapid,accurate,and suitable for the quality control of the tablets.