| 葛根多糖对PC12细胞增殖的损伤作用 |
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| 引用本文： | 宋淑珍,房征宇,田亚平. 葛根多糖对PC12细胞增殖的损伤作用[J]. 军医进修学院学报, 2004, 25(1): 18-20 |
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| 作者姓名： | 宋淑珍 房征宇 田亚平 |
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| 作者单位： | 1. 解放军总医院,生化科,北京,100853
2. 解放军总医院,老年医学研究所,北京,100853 |
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| 基金项目： | ”十五”军队科研基金项目 ( 0 1MB0 72 ) |
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| 摘 要： | 目的 :探讨葛根多糖 (totalpolysaccharideofPueraria ,TPP)对嗜铬细胞瘤细胞PC 12增殖的影响。方法 :用细胞培养的方法检测TPP单独或与H2 O2 一起对PC 12细胞活力的影响 ;MTT法检测细胞活力 ,Griess试剂测定细胞外液中的亚硝酸盐含量 ;次黄嘌呤黄嘌呤氧化酶化学发光体系测定细胞外液中的SOD活性。结果 :0 .0 1～ 1.0mg·ml 1的多糖明显地抑制细胞生长 ,并可增强H2 O2 导致的细胞损伤 (P <0 .0 5 ) ;细胞外液中的SOD活性随多糖的浓度增加而降低 ;其亚硝酸盐产物增多 ,并呈浓度依赖性。结论 :TPP对嗜铬细胞瘤细胞PC 12的增殖具有损伤作用。
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| 关 键 词： | 葛根多糖 PCI2细胞 细胞增殖 TPP 过氧化氢 嗜铬细胞瘤 |
| 文章编号： | 1005-1139(2004)01-0018-03 |
| 修稿时间： | 2003-07-21 |
Polysaccharide of Pueraria induced injury on PC12 cells |
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| SONG Shu-zhen ,FANG Zhen-yu ,TIAN Ya-ping. Polysaccharide of Pueraria induced injury on PC12 cells[J]. Academic Journal of Pla Postgraduate Medical School, 2004, 25(1): 18-20 |
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| Authors: | SONG Shu-zhen FANG Zhen-yu TIAN Ya-ping |
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| Affiliation: | SONG Shu-zhen 1,FANG Zhen-yu 2,TIAN Ya-ping 1 |
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| Abstract: | Objective: To examine the effects of polysaccharide of Pueraria in pheochromocytoma-derived PC12 cells. Methods: cytotoxic effects was assessed by measuring cell viability using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium (MTT) assay. Superoxide dismutase (SOD) was measured by xanthine-hypoxanthinee oxidase chemiluminescence system . nitrite production was measured by a Griess assay. Results: 0.01-1.0mg/ml polysaccharide inhibited cellular viability and strengthened the injury induced by H 2O 2 dose-dependently on PC12 ; with the increase of the concentration of polysaccharide,extracellular SOD was reduced while nitrite production was increased .Conclusion: polysaccharide of Pueraria can resulted in PC12 cells damage . |
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| Keywords: | puerariae thomsonii polysaccharides PC12 cells hydrogen peroxide |
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