吡咯烷二硫氨基甲酸酯对犬缺血-再灌注损伤心肌抗氧化作用的影响

目的：探讨体外循环（CPB）中犬心肌能量代谢和脂质过氧化反应的变化，并用吡咯烷二硫氨基甲酸酯（PDTC）进行干预治疗。方法：将12只犬随机分为对照组（C组，n=6）和PDTC组（P组，n=6），建立CPB心肌缺血再灌注模型。P组于CPB前静脉注射PDTC30mg／kg，C组静脉注射等量生理盐水。分别于CPB前和阻断主动脉60min及开放主动脉60min时，取心肌进行三磷酸腺苷（ATP）、超氧化物歧化酶（SoD）、谷胱甘肽过氧化物酶（GSH—PX）、丙二醛（MDA）含量和线粒体肿胀度（MSD）检测。于CPB前和开放主动脉30min及开放主动脉60min时测定血液动力学指标。结杲：P组在阻断主动脉60min和开放主动脉60min时ATP、SOD、GSH—PX含量均显著高于C组（P〈0．01），而MDA和MSD均显著低于C组（P〈0.01）。P组在开放主动脉30min和开放主动脉60min时血液动力学指标恢复迅速（P〈0．01）。结论：PDTC可明显提高缺血心肌抗氧化能力，改善心肌能量代谢。

Effect of pyrrolidine dithiocarbamate on antioxidation of canine myocardium during ichemia/reperfusion injury

OBJECTIVE: To assess the alterations in myocardial energy metabolism and lipid peroxidation during canine cardiopulmonary bypass (CPB), and to investigate the interventional effects of pyrrolidine dithiocarbamate(PDTC) pretreatment. METHODS: Twelve adult healthy dogs undergoing CPB were randomized into control group (Group C, n=6) and PDTC group(Group P, n=6). In Group P, 30 mg/kg PDTC was administered intravenously before CPB and in Group C animals were given physiological saline instead of PDTC. The contents of adenosine triphosphate (ATP), superoxide dismutase(SOD), glutathione peroxidase(GSH-PX), malondialdehyde (MDA) and mitochondrial swelling degree (MSD) of myocardium were determined before CPB, 60 min after aortic cross-clamping (AC) and 60 min after declamping (DC). Hemodynamics was monitored before CPB, 30 min and 60 min after DC. RESULT: Contents of ATP, SOD and GSH-PX in Group P at 60 min after AC and 60 min after DC were higher than those in Group C (P<0.01). MDA and MSD in Group P at 60 min after AC and 60 min after DC were significantly lower than those in Group C (P<0.01). Hemodynamics of Group P was recovered at 30 min and 60 min after DC. CONCLUSION: Pretreatment with PDTC is effective in improving antioxidation capacity of myocardium and ameliorates myocardial energy metabolism.