健脾化瘀中药对大鼠胃溃疡愈合质量的影响

目的 观察健脾化瘀中药对大鼠消化性溃疡愈合质量的影响，进而阐明其抗胃溃疡的可能机制。方法 采用乙酸注射至大鼠胃浆膜下形成胃渍疡模型，随机分为4组，即空白组(灌服生理盐水)、模型组(灌服生理盐水)、雷尼替丁组(灌服雷尼替丁)、中药组(灌服健脾化瘀中药)。用HE染色法对大鼠愈合性胃溃疡再生黏膜进行定量观察，用放射免疫的方法检测血清及胃黏膜的表皮生长因子(EGF)的含量；用免疫组织化学法检测胃黏膜表皮生长因子受体(EGFR)蛋白表达。结果 中药组再生黏膜厚度高于模型组及雷尼替丁组(P<0．05或P<0．01)，中药组及雷尼替丁组胃黏膜组织的EGF的含量显著高于模型组(P<0．01)，中药组胃黏膜EGFR蛋白表达较模型组增加(P<0．05)。结论 健脾化瘀中药可能是通过EGFR介导来促进胃黏膜上皮细胞增生，抑制胃酸，改善胃黏膜微循环，从而提高胃溃疡愈合质量，发挥抗溃疡作用，

Effect of Chinese Drugs for Jianpi Huayu on Healing Quality of Gastric Ulcer in Rats

ObjectiveTo observe the effect of Chinese drugs of Jianpi Huayu (JPHY, strengthening Pi and dissolving stasis) on healing quality of gastric ulcer and its mechanism. MethodsThe gastric ulcer model was established by subserous injection of ethanoic acid in rats. Rats were randomly divided into 4 groups, the blank group, the model group, the ranitidine (RT) group and the JPHY group. Quantity of regenerative mucosa of healed gastric ulcer was determined using HE stain, epidermal growth factor (EGF) content in serum and stomach mucosa was detected by RIA and epidermal growth factor receptor (EGFR) protein expression was determined by immunohistochemistry. ResultsThickness of regenerated mucosa in the CHM group was higher than that in the model group and the RT group (P<0 05 or P<0 01); EGF content in mucosa in the JPHY group and the RT group was higher than that in the model group (P<0 01) and EGFR protein expression in the JPHY group was higher than that in the model group (P<0 05). ConclusionJPHY could improve the proliferation of epithelial cells, inhibit gastric acid, improve microcirculation of gastric mucosa through the mediation of EGFR, so as to elevate the healing quality of gastric ulcer, display its anti-ulcer action.