| Effect of basic fibroblast growth factor and hyaluronic acid on proliferation of rabbit chondrocytes in vitro |
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| 引用本文： | 沈雁,李斯明,唐毅,钟灿灿,梁佩红,陈鸿辉. Effect of basic fibroblast growth factor and hyaluronic acid on proliferation of rabbit chondrocytes in vitro[J]. 中华创伤杂志(英文版), 2004, 7(1): 42-44 |
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| 作者姓名： | 沈雁 李斯明 唐毅 钟灿灿 梁佩红 陈鸿辉 |
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| 作者单位： | GuangzhouRedCrossHospital,GuangzhouInstituteofTraumaticSurgery,Guangzhou510220,China |
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| 摘 要： | Objective: To investigate the effect of basic fibroblast growth factor (bFGF) and hyaluronic acid (HA) on the proliferation of rabbit chondrocytes in vitro. Methods: Chondrocytes from the knee joints of New Zealand white rabbits were cultured, bFGF or HA or both were added into the culture medium respectively, and the proliferation of the ehondrocytes was measured with MTT 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl-tetra-zolium bromide. (MTT, Sigma, M2128). Results: Basic fibroblast growth factor (10ng/ml) with low concentration of fetal bovine serum in the culture medium promoted the proliferation of chondrocytes significantly, and this effect reached its maximum when concentration of bFGF reached 50ng/ml. HA itself had no effect on the proliferation of chondrocytcs. However, when bFGF was used in combination with HA, especially when the concentration of bFGF was 50-500ng/ml and that of HA was 10-50ng/ml, the effect on the proliferation of chondrocytes was much more than when bFGF or HA was used alone. Conclusions: bFGF can promote the proliferation of chondrocytes. HA, which has no effect on the proliferation of the cells, can maintain a normal growth of chondrocytcs.When bFGF is used in combination with HA, more proliferation is obtained.
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| 关 键 词： | 基质纤维原细胞生长因子 透明质酸 软骨细胞 细胞增殖 作用机制 软骨损伤 |
Edited by SONG Shuang-mingEffect of basic fibroblast growth factor and hyaluronic acid on proliferation of rabbit chondrocytes in vitro |
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| SHEN Yan ,LI Si ming,TANG Yi,ZHONG Can can,LIANG Pei hong and CHEN Hong hui Guangzhou Red Cross Hospital,Guangzhou Institute of Traumatic Surgery,Guangzhou ,China. Edited by SONG Shuang-mingEffect of basic fibroblast growth factor and hyaluronic acid on proliferation of rabbit chondrocytes in vitro[J]. Chinese journal of traumatology, 2004, 7(1): 42-44 |
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| Authors: | SHEN Yan LI Si ming TANG Yi ZHONG Can can LIANG Pei hong CHEN Hong hui Guangzhou Red Cross Hospital Guangzhou Institute of Traumatic Surgery Guangzhou China |
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| Affiliation: | Guangzhou Red Cross Hospital, Guangzhou Institute of Traumatic Surgery, Guangzhou 510220, China |
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| Abstract: | Objective: To investigate the effect of basic fibroblast growth factor (bFGF) and hyaluronic acid (HA) on the proliferation of rabbit chondrocytes in vitro. Methods: Chondrocytes from the knee joints of New Zealand white rabbits were cultured. bFGF or HA or both were added into the culture medium respectively, and the proliferation of the chondrocytes was measured with MTT 3 (4, 5 dimethylthiazol 2 yl) 2, 5 diphenyl tetra zolium bromide. (MTT, Sigma, M2128). Results: Basic fibroblast growth factor (10 ng/ml) with low concentration of fetal bovine serum in the culture medium promoted the proliferation of chondrocytes significantly, and this effect reached its maximum when concentration of bFGF reached 50 ng/ml. HA itself had no effect on the proliferation of chondrocytes. However, when bFGF was used in combination with HA, especially when the concentration of bFGF was 50 500 ng/ml and that of HA was 10 50 ng/ml, the effect on the proliferation of chondrocytes was much more than when bFGF or HA was used alone. Conclusions: bFGF can promote the proliferation of chondrocytes. HA, which has no effect on the proliferation of the cells, can maintain a normal growth of chondrocytes. When bFGF is used in combination with HA, more proliferation is obtained. |
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| Keywords: | Basic fibroblast growth factor Hyaluronic acid Chondrocytes |
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