登革2型病毒粘附人血小板的体外研究初探

目的:了解登革2型病毒(DEN-2)是否能在体外粘附人血小板并制备抗人血小板抗体.方法:密度梯度离心法分离血小板,DEN-2与血小板体外共孵育2 h,间接免疫荧光法分析DEN-2粘附人血小板情况.采用TritonX-100裂解法制备血小板蛋白并经皮下注射免疫BALB/C小鼠获取抗血小板血清,经Western blot、...

A Preliminary Study on Adherence of Type 2 Dengue Virus to Human Platelets in Vitro

Objective:To investigate whether type 2 dengue virus(DEN-2) could adhere to human platelets(PLT) in vitro and to prepare mouse anti-human platelet antibody.Methods: Density gradient centrifugation was used to isolate PLT.DEN-2 was co-cultured with human PLT for 2 hours.DEN-2 adhering to PLT was detected with indirect immunofluorescence assay(IFA).The antiserum against PLT was prepared by subcutaneous injection of PLT proteins extracted with Triton X-100 into BALB/C rats.The specificity of anti-PLT antibodies was identified by Western blot and IFA.Titration of them was examined with indirect ELISA.Results: DEN-2 could adhere to human PLT.The specific anti-human PLT antibodies were obtained by Western blot and identified by IFA.The titration of the antibodies was 1∶12 800 detected by indirect ELISA.Conclusions: DEN-2 could adhere to human PLT in vitro.Specific anti-human PLT antibodies with high titration could be prepared by immunizing BALB/C mice with PLT proteins.These results lay a foundation for further study of PLT immune function in DEN-2 caused infectious diseases.