| B3HM细胞内活性物质对小鼠造血的作用 |
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| 引用本文： | 姜学英,张丽艳,李侠,蔡辉国,宋增璇. B3HM细胞内活性物质对小鼠造血的作用[J]. 肿瘤, 2002, 22(1): 17-19 |
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| 作者姓名： | 姜学英 张丽艳 李侠 蔡辉国 宋增璇 |
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| 作者单位： | 中国医学科学院中国协和医科大学血液学研究所,实验血液学国家重点实验室,天津,300020 |
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| 基金项目： | 国家自然科学基金资助(398702191) |
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| 摘 要： | 目的 观察B3HM细胞条件培养液 (B3HM CM )和细胞匀浆组分对小鼠造血细胞的作用。寻找其中可能存在的诱导造血细胞发生恶性转化的活性物质。方法 将B3HM CM加到小鼠骨髓长期培养体系中 ,定期计数细胞生长情况 ;用B3HM CM或经差速离心法分离的B3HM细胞匀浆组分注射经照射处理的小鼠 ,观察小鼠存活情况和造血细胞的变化。结果 B3HM CM在骨髓培养体系中促巨核细胞增殖 ,在小鼠体内促造血细胞增多。B3HM细胞匀浆第 3组分可诱发小鼠白血病。结论 B3HM细胞可能存在诱导造血细胞发生恶性转化的物质和细胞增殖刺激因子
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| 关 键 词： | B_3HM细胞 条件培养液 骨髓细胞长期培养 造血细胞刺激因子 |
| 修稿时间： | 2001-01-02 |
The effects of B3HM cell produced factors on murine hematopoiesis |
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| JIANG Xueying ZHANG Liyan LI Xia et al.. The effects of B3HM cell produced factors on murine hematopoiesis[J]. Tumor, 2002, 22(1): 17-19 |
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| Authors: | JIANG Xueying ZHANG Liyan LI Xia et al. |
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| Abstract: | Objective: To study the effects of B 3HM CM (condition medium) and B 3HM cell ground fractions on mouse hematopoietic cells and to search for possible factors inducing hematopoietic cell transformation.Methods: The B 3HM CM was added to long term cultures of mouse bone marrow cells,and cell counting monitored the cell growth.The B 3HM CM or fractions of B 3HM C cell ground materials were injected into BALB/c mice,which were irradiated 4.5 Gy by 137 Cs.The mice hematopoietic cell growth and survival conditions were observed.Results: The B 3HM CM stimulated megakaryocyte growth in long term cultures.It also enhanced hematopoiesis in vivo.The third fraction of the B 3HM C cell ground material induced mouse leukemia.Conclusion: The B 3HM C cells produce factors,which stimulate hematopoietic cell proliferation and possibly induce hematopoietic cell transformation. |
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| Keywords: | B 3HM cell Condition medium Bone marrow long term culture Hematopoietic stimulate factor |
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