| 两亲性含IKVAV的肽体内诱导血管生成的研究 |
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| 引用本文： | 宋玉林,郑启新,郭晓东,黄华斌. 两亲性含IKVAV的肽体内诱导血管生成的研究[J]. 组织工程与重建外科, 2008, 4(5): 254-258 |
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| 作者姓名： | 宋玉林 郑启新 郭晓东 黄华斌 |
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| 作者单位： | [1]南昌大学第二附属医院骨科,江西省南昌市330006 [2]华中科技大学同济医学院协和医院骨科,湖北省武汉市430000 |
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| 摘 要： | 目的设计合成含IKVAV的两亲性肽，探讨其体外自组装成三维多孔凝胶及体内诱导血管生成的可行性。方法固相法合成肽．自组装成凝胶，TEM观察凝胶结构。实验组：配制1wt％的肽溶液，pH值为9．0；对照组：配制16．67％的明胶，DH值为7．4。各取1mL，分别注射于大鼠脊柱两侧皮下，术后1周观察全身反应及局部皮肤。2周后取材，固定，常规HE染色及VEGF免疫组织化学染色。结果高效液相频谱仪及质谱仪示肽纯度及分子量分别为95．22％与1438,TEM示凝胶为交织成网状的纳米纤维。术后1周动物存活良好，无全身不良反应，局部皮肤无红肿、无坏死。实验组：1wt％的肽体内自组装成凝胶，血管长入凝胶内部，管腔内可发现红细胞；术后2周，VEGF阳性。对照组：明胶内未发现血管，VEGF阴性。结论本实验合成含IKVAV两亲性肽，在PBS溶液中可自组装成凝胶且体内可诱导新生血管生成。
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| 关 键 词： | 血管生成 自组装 凝胶 |
Research on Angiogenesis Induced with IKVAV-containing Amphiphile Peptide in Vivo |
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| SONG Yulin ZHENG Qixin GUO Xiaodong HUANG Huabing. Research on Angiogenesis Induced with IKVAV-containing Amphiphile Peptide in Vivo[J]. , 2008, 4(5): 254-258 |
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| Authors: | SONG Yulin ZHENG Qixin GUO Xiaodong HUANG Huabing |
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| Affiliation: | SONG Yulin ZHENG Qixin GUO Xiaodong HUANG Huabing.1 Department of Orthopaedics,The Second Affiliated Hospital,Nanchang University,Nanchang 330006,China,2 Department of Orthopedics,Union Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430022,Chino. |
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| Abstract: | Objective The amphiphile peptide containing IKVAV sequence was fabricated and subcutaneously implanted into rat backbone.The angiogenesis induced with peptide in vivo were explored.Methods The peptide was synthesized and self-assembled into hydrogel whose structure was confirmed with TEM.1 millilitre of 1% peptide (EG) and 16.67% gelatin (CG) were injected subcutaneously beside rat backbone.The systemic response and local skin were observed one week after injection.The specimens were harvested two weeks lat... |
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| Keywords: | Angiogenesis Self-assembly Hydrogel |
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