| 采用膜片钳技术记录心室肌细胞离子流 |
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| 引用本文： | 徐浩东,蒋文平.采用膜片钳技术记录心室肌细胞离子流[J].中国心脏起搏与心电生理杂志,1993(1). |
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| 作者姓名： | 徐浩东 蒋文平 |
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| 作者单位： | 苏州医学院附属第一医院心血管研究室，苏州医学院附属第一医院心血管研究室 苏州 215006，苏州 215006 |
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| 摘 要： | 本文报告了心室肌细胞分离方法,采用膜片钳技术,成功地记录了细胞膜钾、钙、钠离子流,初步显示背景钾离子通道活性的电压依赖性,钙、钠离子通道活性为电压和时间依赖性,钠离子通道开放速率最快,电压在-30mV。钠离子流达峰电流仅需1ms,其活性也表现电压和时间依赖性。
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| 关 键 词： | 单个心室肌细胞 膜片钳全细胞记录 背景钾离子流 钙离子流 钠离子流 |
Recording Currents of Potassium, Calcium, Sodium of Ventricular Cells Using Patch-Clamp Technique |
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| Xu Haodong,Jiang Wenping.Recording Currents of Potassium, Calcium, Sodium of Ventricular Cells Using Patch-Clamp Technique[J].Chinese Journal of Cardiac Pacing and Electrophysiology,1993(1). |
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| Authors: | Xu Haodong Jiang Wenping |
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| Abstract: | The method of isolating ventricular cells was reported in this paper and currents of potassium, calcium, sodium of cell membranes were recorded successfully with patch-clamp technique. The results indicated that the activity of background potassium channels was voltagedependent, the activity of calcium and sodium channels was voltage-dependent and time-dependent. Open rate of sodium channels was most fast. And sodium current took one millisecond achieving the largest current at — 30mV. |
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| Keywords: | Single ventricular cell Patch-clamp whole cell recording Background potassium current Calcium current Sodium current |
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