Effect of Melatonin on Proliferative Activity and Apoptosis in Spermatogenic Cells in Mouse under Chemotherapy |
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Authors: | Fahimeh Mohamad Ghasemi Masoumeh Faghani Sina Khajehjahromi Mohamadhadi Bahadori Ebrahim Nasiri E Masoud Hemadi |
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Affiliation: | [1]Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran [2]Histotechnique Laboratory, Histology Division, Anatomical Sciences Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran [3]Student Research Committee, Guilan University of Medical Sciences, Rasht, lran [4]Anatomy Department, Faculty of Medicine, Joundishapur University, Ahwaz, Iran |
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Abstract: | Objective To investigate the possible protective role of melatonin on the proliferativeactivity, and apoptosis of germ cells in chemotherapy-induced spermiotoxicity.Methods Male adult NMRI mice were divided into four groups. Then each group was divided into two subgroups of a and b. Group A (control): mice received vehicle (1% ethanol)for 5 d. Group B (busulfan): mice received a single dose of 20 mg/kg busulfan. Group C (melatonin): mice received melatonin (10 mg/kg) for 5 d. Group D (busulfan+melatonin): mice received a 5-day course of melatonin (10 mg/kg) following an initial dose of busulfan (20 mg/kg). Evaluations were made using bromodeoxyuridine (BrdU) labeling and in situ TUNEL assay after 5 d (subgroup a) or 35 d (subgroup b). Results Busulfan-treated mice both in subgroups a and b, showed a significant increase in the number of apoptotic cells (P<0.01) and decrease in BrdU labeling index (P<0.01) compared with controls. Melatonin in group C significantly decreased BrdU labeling index compared with the control (P <0.01). Melatonin in group D significantly reduced apoptosis rate and increased BrdU labeling index compared with group B.Conclusion Melatonin may have a protective effect against busulfan-induced testicular damage, partly by decreasing of apoptosis and alteration in proliferative activity of germ cells. |
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Keywords: | melatonin busulfan testis apoptosis proliferative activity sperm parameters |
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