| 前列腺癌细胞内冬凌草甲素的浓度动态变化 |
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| 引用本文： | 袁晓勇,赵永星,孙倩,刘丹杏. 前列腺癌细胞内冬凌草甲素的浓度动态变化[J]. 中国医药工业杂志, 2011, 42(8) |
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| 作者姓名： | 袁晓勇 赵永星 孙倩 刘丹杏 |
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| 作者单位： | 1. 南阳市中心医院,河南南阳,473009;郑州大学药学院,河南郑州,450001
2. 郑州大学药学院,河南郑州,450001 |
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| 基金项目： | 河南省科技攻关计划项目，2009年河南省高等学校青年骨干教师资助计划 |
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| 摘 要： | 建立了高效液相色谱法测定人前列腺癌PC-3细胞内的冬凌草甲素.采用C<,18>色谱柱,以甲醇-水(53:47)为流动相,检测波长238 nm.细胞内冬凌草甲素在10～300 ng/ml浓度范围内线性关系良好,绝对回收率为77.79%～81.32%,RSD为3.29%～4.09%;日内和日间RSD均小于8%.人前列腺癌PC-3细胞与冬凌草甲素溶液或冬凌草甲素纳米粒孵育后,采用该法测定胞内药物浓度动态变化过程.结果表明,纳米粒可显著提高细胞内冬凌草甲素的聚集并减慢其消除.
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| 关 键 词： | 冬凌草甲素 前列腺癌细胞 纳米粒 高效液相色谱 测定 |
Intracellular Dynamic Variation of Oridonin in Prostate Cancer Cells |
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| YUAN Xiaoyong,ZHAO Yongxing,SUN Qian,LIU Danxing. Intracellular Dynamic Variation of Oridonin in Prostate Cancer Cells[J]. , 2011, 42(8) |
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| Authors: | YUAN Xiaoyong ZHAO Yongxing SUN Qian LIU Danxing |
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| Affiliation: | YUAN Xiaoyong~(1,2),ZHAO Yongxing~(2*),SUN Qian~2,LIU Danxing~2 (1.Center Hospital of Nanyang,Nanyang 473009,2.School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001) |
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| Abstract: | An HPLC method was established for the determination of oridonin in human prostate cancer cells (PC-3).A C_(18) column was used with the mobile phase of methanol-water(53:47) at the detection wavelength of 238 nm.The calibration curve was linear in the concentration range of 10-300 ng/ml.The absolute recoveries were 77.79%-81.32%,with RSDs of 3.29%-4.09%.The inter-and intra-day RSDs were less than 8%.PC-3 Cells were treated with oridonin solution or oridonin nanoparticles and the intracellular dynamic varia... |
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| Keywords: | oridonin prostate cancer cell nanoparticle HPLC determination |
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