两种拟除虫菊酯类农药抗雄激素活性的Hershberger实验研究

目的 研究两种拟除虫菊酯类农药氯氰菊酯和甲氰菊酯在体内的抗雄激素作用.方法 6周龄雄性SD大鼠去势后恢复一星期,按体重随机分为8组,每组6只,包括阴性对照组,氯氰菊酯与甲氰菊酯低、中、高剂量组,阳性对照组.各组动物均皮下注射丙酸睾酮(TP)100μg/(kg·d)连续7 d.阴性对照组、两种拟除虫菊酯农药组和阳性对照组分别皮下注射给予花生油、氯氰菊酯[45、90、180mg/(kg·d)]、甲氰菊酯[10、20、40mg/(kg·d)]、氟他胺(20mg/kg),连续7 d,观察大鼠性附属组织及血清激素水平的变化.结果 两种拟除虫菊酯类农药各剂量组与阴性对照组比较,大鼠性附属器官精囊腺、背侧前列腺、腹侧前列腺和提肛肌重量,以及大鼠血清睾酮(T)、促卵泡激素(FSH)和黄体生成素(LH)水平差异均无统计学意义.阳性对照组与阴性对照组比较,大鼠各性附属器官重量均降低,大鼠LH水平升高,差异均具有统计学意义.结论 氯氰菊酯和甲氰菊酯对去势雄性大鼠的性附属器官重量和血清T、FSH和LH水平无明显影响,未显示出抗雄激素活性.

Abstract：

Objective To observe the anti-androgenic activity of two pyrethroids (cypermethrin and fenpropathrin) in vivo. Methods SD rats aged 6 weeks were castrated ,and then housed for one week. The rats were randomly divided into groups, six in each: negative control group, cypermethrin and fenpropathrin group, positive control group. The rats were subcutaneously injected with testosterone propionate (TP) 100 μg/(kg·d) for 7 consecutive days. Peanut oil, cypermethrin [ 45, 90, 180 mg/(kg·d)], fenpropathrin[10, 20, 40 mg/ (kg·d) ], fiutamide[ 20 mg/(kg·d) ] were subcutaneously injected for 7 consecutive days at the same time. The sex accessory glands and serum hormone were detected. Results Compared with the negative group, no significant differences were observed in the sex accessory glands/tissues (seminal vesicles with coagulating glands, dorsolateral prostate, the ventral prostate and levator ani muscles) and the levels of serum hormones of the pyrethroids groups. Compared with the negative group, the masses of all the sex accessory glands/tissues decreased significantly in the positive group . Compared with the negative group, the level of LH increased significantly in the positive group. Conclusion No obvious anti-androgenic effects induced by cypermethrin and fenpropathrin in castrated SD rats has been observed.

Anti-androgenic Activity of Cypermethrin and Fenpropathrin in Hershberger Assays

Objective To observe the anti-androgenic activity of two pyrethroids (cypermethrin and fenpropathrin) in vivo. Methods SD rats aged 6 weeks were castrated ,and then housed for one week. The rats were randomly divided into groups, six in each: negative control group, cypermethrin and fenpropathrin group, positive control group. The rats were subcutaneously injected with testosterone propionate (TP) 100 μg/(kg·d) for 7 consecutive days. Peanut oil, cypermethrin [ 45, 90, 180 mg/(kg·d)], fenpropathrin[10, 20, 40 mg/ (kg·d) ], fiutamide[ 20 mg/(kg·d) ] were subcutaneously injected for 7 consecutive days at the same time. The sex accessory glands and serum hormone were detected. Results Compared with the negative group, no significant differences were observed in the sex accessory glands/tissues (seminal vesicles with coagulating glands, dorsolateral prostate, the ventral prostate and levator ani muscles) and the levels of serum hormones of the pyrethroids groups. Compared with the negative group, the masses of all the sex accessory glands/tissues decreased significantly in the positive group . Compared with the negative group, the level of LH increased significantly in the positive group. Conclusion No obvious anti-androgenic effects induced by cypermethrin and fenpropathrin in castrated SD rats has been observed.