甲醛吸入急性肺水肿大鼠心、肺、脑、肝和血清蛋白浓度及一氧化氮合酶活力变化

目的 研究甲醛吸人性急性肺水肿模型大鼠心、肺、脑、肝和血清蛋白浓度及一氧化氮合酶(NOS)活力的变化,为研究此类肺水肿提供实验依据.方法 健康成年清洁级SD大鼠18只随机分为对照组和模型组,在染毒箱内以"静态+喷雾"吸入40%甲醛2 h复制急性肺水肿模型,8 h后眼球采血并处死,按试剂盒说明书测定血清及心、肺、脑、肝组织匀浆的蛋白浓度和NOS活力,并计算心肺系数、心指数和肺指数,肺组织作常规病理检查.对照组将甲醛换为生理盐水,处理方法同模型组.结果 与生理盐水对照组比较,模型组心肺系数显著增大(P<0.05),病理切片显示模型组出现肺组织间隙增大、大量有核细胞浸润等肺水肿改变;血清、心脏、脑和肺匀浆蛋白浓度显著增加(P<0.05或P<0.01),肝蛋白浓度差异无统计学意义(P>0.05);血清、心、肺、脑、肝NOS活力无统计学意义(均P>0.05).结论 甲醛吸入性急性肺水肿大鼠血清、心脏、脑和肺蛋白含量增加.NOS活力变化不大.

Abstract：

Objective To study the changes of the protein concentration (Cpro) and nitric oxide synthase (NOS) of serum, heart, lungs, brain and liver in acute pulmonary edema rats model induced by formaldehyde inhalation, to provide data for studying in acute pulmonary edema animal model induced by formaldehyde inhalation. Methods Eighteen SD female rats were randomly divided into the control group and the model group, and the model group rats were inhaled vaporous formaldehyde in a self-made cabinet for 2 hours and were sacrificed 8 hours later. The blood sampling, lungs, heart, brain and liver were collected and lungs/ heart index, heart/body weight index and lungs/body weight index were calculated, NOS were detected with NOS kit and protein determination kit, and the pathological examination of lungs tissue was done. Rats of control group were treated as the same as model group, but formaldehyde was replaced by normal saline. Results Compared with the control, lungs/heart index increased significantly (P<0.05) in the model rats, the lungs pathological examination showed that pulmonary tissue space broadening and filled with lots of nucleated cells in the model rats. Protein concentration of serum, heart, brain and lungs increased significantly (P<0.05 or P<0.01) in model rats, but no significant change was seen in NOS of serum, brain, heart, liver and lungs (P>0.05). Conclusion In acute pulmonary edema rats induced by formaldehyde inhalation, the protein concentration of serum, heart, brain and lungs tissues increase significantly, NOS does not change obviously.

Changes of Protein Concentration (Cpro) and Nitric Oxide Synthase(NOS) of Serum, Heart, Lungs, Brain and Liver in Acute Pulmonary Edema Rats Model Induced by Formaldehyde Inhalation

Objective To study the changes of the protein concentration (Cpro) and nitric oxide synthase (NOS) of serum, heart, lungs, brain and liver in acute pulmonary edema rats model induced by formaldehyde inhalation, to provide data for studying in acute pulmonary edema animal model induced by formaldehyde inhalation. Methods Eighteen SD female rats were randomly divided into the control group and the model group, and the model group rats were inhaled vaporous formaldehyde in a self-made cabinet for 2 hours and were sacrificed 8 hours later. The blood sampling, lungs, heart, brain and liver were collected and lungs/ heart index, heart/body weight index and lungs/body weight index were calculated, NOS were detected with NOS kit and protein determination kit, and the pathological examination of lungs tissue was done. Rats of control group were treated as the same as model group, but formaldehyde was replaced by normal saline. Results Compared with the control, lungs/heart index increased significantly (P<0.05) in the model rats, the lungs pathological examination showed that pulmonary tissue space broadening and filled with lots of nucleated cells in the model rats. Protein concentration of serum, heart, brain and lungs increased significantly (P<0.05 or P<0.01) in model rats, but no significant change was seen in NOS of serum, brain, heart, liver and lungs (P>0.05). Conclusion In acute pulmonary edema rats induced by formaldehyde inhalation, the protein concentration of serum, heart, brain and lungs tissues increase significantly, NOS does not change obviously.