自体血小板分离在心血管手术中的应用

体外循环(CPB)期间,由于血小板激活和α-颗粒释放,导致血小板减少和功能下降。CPB前,利用自体血小板分离技术可将部分血小板从患者全血中分离出来制成富血小板血浆(platelet-richplasma,PRP),PRP可在术后回输,以达到血小板数量和功能的双重保护,另一方面可将PRP制备成自体血小板胶(autologousplateletgel,APG),APG中含有丰富的生长因子,并且具有足够的抗张强度和粘性,因此可起到术中止血、封闭伤口、促进胸骨及伤口愈合的作用。

Application of Autologous Plateletpheresis Technology in Cardiovascular Surgery

Cardiopulmonary bypass(CPB) is associated with thrombocytopenia and platelet dysfunction. The primary cause of acquired platelet defect is thought to be activation and release of alpha granules during CPB. Before CPB, platelet-rich plasma (PRP) was prepared by obtaining the required amount of patient's whole blood by autologous plateletpheresis. PRP could be reinfused after operation in order to protect the function and quantities of the platelets. On the other hand, PRP could be made into autologous platelet gel (APG). APG contains supraphysiologic amounts of growth factors, and has adequate tensile strength and adhesive ability. Therefore, it can be used for hemostasis in operation, sealing wound and enhancing incision or dehiscent sternal wounds healing.