Vascularization of embryonic adrenal gland grafted onto chorioallantoic membrane |
| |
Authors: | Mirella Bertossi Daniela Virgintino Pierre Coltey Mariella Errede Lucia Mancini L. Roncali |
| |
Affiliation: | (1) Istituto di Anatomia Umana Normale, Istologia ed Embriologia, Facoltà di Medicina, Università di Bari, Bari, Italy Tel.: +39-80-5478309; Fax: +39-80-5478309; e-mail: roncali@histology.uniba.it, IT;(2) Institut d’Embryologie Cellulaire et Moléculaire, Centre National de la Recherche Scientifique, Collège de France, Nogent-sur-Marne Cedex, France, FR |
| |
Abstract: | Vascularization and endothelial phenotype expression were analysed in embryonic adrenal tissue grafted onto chorioallantoic membrane (CAM), by means of routine light microscopy and immunocytochemical staining, and of electron microscopy. Adrenal gland tissue from chick or quail embryos (donors) was grafted onto CAMs of chick or quail embryos (host). Vessels of chick origin were discriminated from those of quail origin by monoclonal antibodies, anti-MB1, specific for quail endothelial and haemopoietic cells, and QCPN, which labels quail cell nuclei. Vessels of adrenal type were distinguished from those of CAM-type by their ultrastructural endothelial phenotype – porous in the former and continuous in the latter. The observations carried out 6 days after implantation indicate that the adrenal gland develops and differentiates according to a virtually normal histological pattern. As regards the adrenal and CAM vascularization, the grafting procedure elicits angiogenic events consisting in the formation of peripheral anastomoses between the graft and the CAM original microvasculature and in new-growth of vessels from the CAM into the grafted tissue and vice versa. As to the endothelial phenotype, the ultrastructural results demonstrate that besides its own native vasculature, the adrenal tissue contains vessels with continuous endothelium and the CAM mesenchyme is supplied by adrenal-type, fenestrated vessels. Accepted: 27 April 1998 |
| |
Keywords: | Chorioallantoic membrane Embryonic grafts Adrenal gland Vascularization Endothelial phenotype |
本文献已被 SpringerLink 等数据库收录! |
|