血清肝素抗凝血浆对化学法检测微量元素的影响

目的探讨不同处理方式的血清及不同肝素浓度抗凝血浆对化学法检测微量元素的影响。方法以临床工作中可能出现的5种肝素浓度抗凝血浆与快速处理的血清标本在相同条件下配对检测微量元素,并与常规处理的血清标本进行比较。结果不同浓度肝素抗凝血浆与快速处理的血清比较,铜(Cu)、镁(Mg)、锌(Zn)、铁(Fe)和总钙(TCa)差异无统计学意义(P>0.05),磷(P)差异有统计学意义(P<0.01);快速与常规2种方式处理的血清比较:Mg、Zn和Fe差异有统计学意义(P<0.05,P<0.01);不同浓度肝素抗凝血浆与常规处理的血清比较除TCa外差异均有统计学意义(P<0.05,P<0.01);不同浓度肝素抗凝血浆之间比较差异无统计学意义(P>0.05)。结论如无条件快速处理血清,化学法检测微量元素应尽量使用肝素化抗凝血浆;肝素抗凝血浆的应用要充分考虑到个别项目如P的局限性;肝素抗凝浓度应按照《全国临床检验操作规程》执行,各实验室还应建立肝素抗凝血标本微量元素化学法检测指标的参考范围。

The effect of serum with quick treatment and plasma with different concentration of heparin on the trace element determination

Objective To investigate the effect of serum with quick treatment and plasma with different concentration of heparin on the determination of trace element. Methods Trace elements were determined in plasma samples with 5 different concentrations of heparin and in the serum samples treated with quick technique.Results were compared with that of normal serum sample. Results The levels of copper(Cu),magnesium(Mg),zinc(Zn),iron(Fe) and total caleium(TCa) had no significant difference between plasma and serum samples of quickly treating technique(P>(0.05),) but the level of phosphorus(P) had significant difference(P<0.01).The levels of Mg,Zn and Fe had significant difference between the normal serum and the serum samples of quickly treating technique(P<0.05,P<0.01),and the levels of all trace elements had significant difference between the plasma and normal serum samples except TCa(P<0.05,P<0.01),but the levels of all trace elements had no significant difference between the plasma samples with 5 different concentrations of heparin(P>0.05). Conclusions The plasma sample anticoagulated with heparin is well-suited for the determination of trace elements,but some factors must be taken into account,particularly the limited chemical items such as P.It is equally important that the normal reference range in blood samples with heparin should be established.