| 低强度脉冲超声波对体外培养人退变腰椎间盘细胞的作用 |
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| 引用本文： | 刘清和,周君琳,陈亚平,孙鹏,海涌.低强度脉冲超声波对体外培养人退变腰椎间盘细胞的作用[J].中国骨与关节损伤杂志,2009,24(4). |
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| 作者姓名： | 刘清和 周君琳 陈亚平 孙鹏 海涌 |
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| 作者单位： | 1. 首都医科大学附属北京朝阳医院骨科,北京市,100020
2. 兵器工业北京北方医院 |
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| 基金项目： | 首都医学发展科研基金项目(2007-2058) |
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| 摘 要： | 目的 探讨低强度脉冲超声波(LIPUS)对体外培养人退变腰椎间盘细胞的作用.方法 体外培养人退变髓核细胞,台盼蓝染色法测定活细胞率.将每份髓核细胞样本分为对照组和LIPUS组.应用LIPUS每天作用20 min,作用后第0、2、4、8天,采用免疫组化法测定Ⅱ型胶原水平,采用酶联免疫吸附法检测聚集蛋白聚糖(aggrecan)水平.结果 体外培养人退变髓核细胞的活细胞率为90%～95%.LIPUS干预后第2、4、8天,LIPUS组Ⅱ型胶原的合成和aggrecan含量均较相应时间点对照组显著增加(P＜0.05).结论 LIPUS能够通过促进人退变髓核细胞合成Ⅱ型胶原和蛋白多糖防治椎间盘退变.
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| 关 键 词： | 低强度脉冲超声波 椎间盘退变 髓核细胞 |
Effect of Low-intensity Pulsed Ultrasound on Human Degenerative Lumbar Intervertebral Disc Cells in Vitro |
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| Liu Qinghe,Zhou Junlin,Chen Yaping,et al..Effect of Low-intensity Pulsed Ultrasound on Human Degenerative Lumbar Intervertebral Disc Cells in Vitro[J].Chinese Journal of Bone and Joint Injury,2009,24(4). |
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| Authors: | Liu Qinghe Zhou Junlin Chen Yaping |
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| Institution: | Liu Qinghe,Zhou Junlin,Chen Yaping,et al.Department of Orthopedics,Beijing Chaoyang Hospital Affiliated to Capital Medical Science University,Beijing,100020 |
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| Abstract: | Objective To study the effect of low-intensity pulsed ultrasound(LIPUS) on human degenerative lumbar intervertebral disc cells in vitro.Methods The human degenerative lumbar intervertebral disc cells were isolated from degenerated disc and cultured in DMEM/F12 in vitro.The viability of cells were determined by trypan blue exclusion test.They were then divided into control and LIPUS groups.Cells in LIPUS groups were treated by LIPUS for 20 min daily.The type II collagen and aggrecan levels were detected by i... |
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| Keywords: | Low-intensity pulsed ultrasound Intervertebral disc degeneration Nucleus pulposus cell |
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