| 中国麻疹病毒疫苗株与野毒株鉴别方法的建立 |
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| 引用本文： | 周剑惠,;王爽,;陈超,;常新,;刘桂艳,;桃育晖,;潘希平,;张燕,;许松涛,;许文波. 中国麻疹病毒疫苗株与野毒株鉴别方法的建立[J]. 中国疫苗和免疫, 2009, 0(4): 310-315 |
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| 作者姓名： | 周剑惠, 王爽, 陈超, 常新, 刘桂艳, 桃育晖, 潘希平, 张燕, 许松涛, 许文波 |
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| 作者单位： | [1]吉林省疾病预防控制中心,吉林省卫生厅病毒重点实验室,长春130021; [2]长春市疾病预防控制中心,吉林长春130033; [3]长春市二道区疾病预防控制中心,吉林长春130031; [4]中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室,世界卫生组织西太平洋区麻疹参比实验室,北京100050 |
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| 基金项目： | 吉林省科学技术发展计划项目（合同编号：200705492）; 吉林省卫生厅病毒重点实验室科研课题 |
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| 摘 要： |
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| 关 键 词： | 麻疹病毒 疫苗株 野毒株 逆转录-聚合酶链反应 限制性片段长度多态性分析 |
The Establishment of Method for Identifying China Vaccine Strains and Wild Strains of Measles Virus |
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| Affiliation: | ZHOU Jian-hui, WANG Shuang, CHEN Chao, et al. (Center for Disease Control and Prevention of Jilin Province, Key Laboratory of Health Bureau of Jilin Province, Changchun 130062, Jilin, China) |
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| Abstract: | Objective To establish a simple and quick method for identifying China vaccine strains and wild strains of Measles Virus. Methods To search the enzyme site in Hemagglutinin gene of measles virus for different domestic vaccine strains and wild strains of measles virus,and design the RT-PCR primer within the range covering the enzyme site,and then to confi rm the specifi city and sensibility of the RT-PCR method,and then identify the RT-PCR product by RFLP. Results The one-step RT-PCR method is sensitive,the measles virus of 4.64 TCID50 can be detected at least. No positive bands can be found in the non-measles virus strains,it means that the RT-PCR method has good specifi city,the PCR products of Chian measles vaccine strains of Shang-191 and Chang-47 were all cut into two fragments （287bp and 151bp） by Afi II,but two measles wild virus strains can＇t be cut by A？ II. Conclusion The RT-PCR-RFLP method which we established is a rapid and simple method for identifying China vaccine strain .and wild strain. |
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