| 乙型肝炎病毒标志参比系统的建立 |
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| 引用本文： | 张勇,;王锋,;马景臣,;张文英,;余陶,;赵守军,;毕胜利. 乙型肝炎病毒标志参比系统的建立[J]. 中国疫苗和免疫, 2009, 0(5): 385-387 |
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| 作者姓名： | 张勇, 王锋, 马景臣, 张文英, 余陶, 赵守军, 毕胜利 |
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| 作者单位： | [1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052; [2]河北省疾病预防控制中心,石家庄050021; [3]Department of Radiology, University of California, San Francisco 94143, USA |
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| 基金项目： | 卫生部全国乙型病毒性肝炎血清流行病学调查专项资金 |
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| 摘 要： | 目的建立乙型肝炎（乙肝）病毒标志参比系统。方法选择不适合临床使用的血浆155份为预备参比品,使用美国Abbott公司生产的微粒子酶免疫测定法乙肝五项检测试剂,重复检测每份预备参比品各2次。凡2次检测结果同为阳性或阴性者,入选参比系统;凡检测结果为一阴一阳者,剔除。结果乙肝病毒表面抗原参比系统有153份血浆入选;抗乙肝病毒表面抗原抗体、抗乙肝病毒核心抗原抗体、抗乙肝病毒e抗原抗体各有154份血浆入选;乙肝病毒e抗原因只有4份阳性血浆,不能构成参比系统。结论通过使用性能出众的试剂2次重复检测,确定足够数量的阳性和阴性参比品,可以构成参比系统。使用参比系统要注意其适用范围,避免产生错误。
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| 关 键 词： | 乙型肝炎病毒标志 参比系统 |
Establishment of Hepatitis B Virus Marker Panels |
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| Affiliation: | ZHANG Yong, WANG Feng, MA Jing-chen, et al. (State Key Laboratory for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Beijing 100052, China.) |
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| Abstract: | Objective To establish HBV marker panels. Methods 155 blood plasma specimens were selected and each was detected twice for 5 HBV markers with MEIA kits made by Abbott. Any plasma that had 2 positive or negative results were enrolled in the HBV marker panels and others were excluded. Results 153 plasma were enrolled in the HBsAg panel and 154 were enrolled in anti-HBs, anti-HBc and anti-HBe panels respectively. Panel of HBeAg was not established because that there were only 4 positives. Conclusion HBV marker panels can be established by screening enough blood plasma specimens with high accurate kits for more than one time. The panels should be used in a limited extension to avoid unnecessary mistakes. |
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| Keywords: | Hepatitis B Virus markers Panel |
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