Studies on the Relationship between Urokinase Plasminogen Activator(uPA)and Human Sperm Motility

Plasminogenactivators(PAs)arehighlyselectivetrypsin--likeserineproteaseabletoconverttheinactiveplasminogen,azymogenpresentinmostextracellularfluids,intoplasmin,alsoatrypsin--likeproteaseofbroadspecificity'].SeminalplasmalevelsofPAsinmenshowup60to100foldhigherthaninbloodplasma'].ThetwoknownPAs,urokinase--type(uPA)andtissue-type(iPA)aresecretedbyalargenumberofcellsandarethoughttoexhibitmultiplefunctionsinextracellularproteolysisaccompanyingtissueremodelingandcellmigration']'Duetotheseprop…

Studies on the relationship between urokinase plasminogen activator (uPA) and human sperm motility

Summary To clarify the role of urokinase plasminogen activator (uPA) in the mechanisms of regulating sperm motility and the ability of fertilizing, we investigated the quantities and activities of uPA in human seminal plasma and on the membrane of spermatozoa. Semens were harvested from 22 infertile patients with asthenospermia and 20 healthy fertile men according to WHO standards. To quantify the membrane-bound uPA in the samples, polyclonal antibodies against human urokinase were employed by means of a sandwich ELISA. The uPA activities in seminal plasma and on the surface of spermatozoa were determined using A-garose-Fibrine-Plate method and the experiment of immunological identification with polyclonal antibodies against urokinase. In lysates of spermatozoa, significantly lower levels of uPA (23. 1±7. 35 mu/10⁶ cells) and uPA activity (5. 13±3. 85 mu/10⁶ cells) were found in patient group as compared to healthy fertile men exhibiting normospermia (29. 89±9. 40 mu/10⁶ cells and 10. 17±6. 18 mu/10⁶ cells). In seminal plasma, uPA activity in patient group (2134 ± 1581. 3 IU/L) was also found significantly lower than that of normal group (3365 ± 1859. 5 IU/ L). Positive correlations were observed between sperm motility and uPA quantities (r=0, 48,P<0. 005), as well as with uPA activities (r = 0. 45,P<0. 005). Thus, it is inferred that membrane associated uPA on human spermatozoa may be related directly to sperm motility and fertility.