Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system |
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| Authors: | Shrestha Nabin K Tuohy Marion J Hall Gerri S Isada Carlos M Procop Gary W |
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| Affiliation: | Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA. |
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| Abstract: | ![]()
One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for methicillin resistance in coagulase-negative staphylococci. |
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